Investigating the role of DNA damage in tobacco smoking-induced spine degeneration |
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| Authors: | Luigi A. Nasto Kevin Ngo Adriana S. Leme Andria R. Robinson Qing Dong Peter Roughley Arvydas Usas Gwendolyn A. Sowa Enrico Pola James Kang Laura J. Niedernhofer Steven Shapiro Nam V. Vo |
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| Affiliation: | 1. Department of Orthopaedic Surgery, Ferguson Laboratory for Orthopaedic Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA;2. Department of Orthopaedic Surgery, Catholic University of Rome School of Medicine, “A. Gemelli” University Hospital, l.go Agostino Gemelli 8, 00168 Roma, Italy;3. Department of Medicine, University of Pittsburgh School of Medicine, 3550 Terrace St, Pittsburgh, PA 15213, USA;4. Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA;5. McGill Scoliosis and Spine Group, Genetics Unit, Shriners Hospital for Children, Montreal, Quebec H3G 1A6, Canada;6. Department of Orthopaedic Surgery of UPMC, Stem Cell Research Center, Pittsburgh, PA 15261, USA;7. Department of Physical Medicine and Rehabilitation, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA;8. Department of Metabolism and Aging, The Scripps Research Institute, 130 Scripps Way #3B3, Jupiter, FL 33458-5284, USA;1. University of Washington School of Medicine, Department of Anesthesiology and Pain Medicine, Seattle, WA 98102, USA;2. Stanford Systems Neuroscience and Pain Laboratory, Department of Anesthesia, Perioperative, and Pain Medicine, Stanford University School of Medicine, Palo Alto, CA, USA;3. Department of Psychology, University of Alabama at Birmingham, 1300 University Blvd., Room 415, Birmingham, AL 35294, USA;1. Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL, USA;2. Universidade Federal do Vale do São Francisco, Petrolina, PE, Brazil;1. Center for Innovation in Immunoregulative Technology and Therapeutics, Graduate School of Medicine, Kyoto University, Kyoto, 606-8501, Japan;2. Department of Health Science and Technology, Aalborg University, Aalborg, 9220, Denmark;1. OMV AG, Corporate Health Management, A-1020 Vienna, Trabrennstr. 4-6, Austria;2. Austrian Academy for Occupational Health & Prevention, President, A-3400 Klosterneuburg, Kierlinger Str. 87, Austria;1. AP-HP, EMS (Samu92), University Hospital of West Suburb of Paris, Poincare Site, F92380 Garches, France;2. AP-HP, Occupational Health Unit, University Hospital of West Suburb of Paris, Poincare Site, F92380 Garches, France;1. AP-HP, EMS (Samu92), University Hospital of West Suburb of Paris, Poincare Site, F92380 Garches, France;2. AP-HP, Occupational Health Unit, University Hospital of West Suburb of Paris, Poincare Site, F92380 Garches, France;3. Univ Versailles St-Quentin, F-78035, Versailles, France;4. Inserm, UMS 011 UMR1168, F-94807, Villejuif, France |
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| Abstract: | ![]()
Background contextTobacco smoking is a key risk factor for spine degeneration. However, the underlying mechanism by which smoking induces degeneration is not known. Recent studies implicate DNA damage as a cause of spine and intervertebral disc degeneration. Because tobacco smoke contains many genotoxins, we hypothesized that tobacco smoking promotes spine degeneration by inducing cellular DNA damage.PurposeTo determine if DNA damage plays a causal role in smoking-induced spine degeneration.Study designTo compare the effect of chronic tobacco smoke inhalation on intervertebral disc and vertebral bone in normal and DNA repair-deficient mice to determine the contribution of DNA damage to degenerative changes.MethodsTwo-month-old wild-type (C57BL/6) and DNA repair-deficient Ercc1?/Δ mice were exposed to tobacco smoke by direct inhalation (4 cigarettes/day, 5 days/week for 7 weeks) to model first-hand smoking in humans. Total disc proteoglycan (PG) content (1,9-dimethylmethylene blue assay), PG synthesis (35S-sulfate incorporation assay), aggrecan proteolysis (immunoblotting analysis), and vertebral bone morphology (microcomputed tomography) were measured.ResultsExposure of wild-type mice to tobacco smoke led to a 19% increase in vertebral porosity and a 61% decrease in trabecular bone volume. Intervertebral discs of smoke-exposed animals also showed a 2.6-fold decrease in GAG content and an 8.1-fold decrease in new PG synthesis. These smoking-induced degenerative changes were similar but not worse in Ercc1?/Δ mice.ConclusionsShort-term exposure to high levels of primary tobacco smoke inhalation promotes degeneration of vertebral bone and discs. Disc degeneration is primarily driven by reduced synthesis of proteoglycans needed for vertebral cushioning. Degeneration was not exacerbated in congenic DNA repair-deficient mice, indicating that DNA damage per se does not have a significant causal role in driving smoke-induced spine degeneration. |
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| Keywords: | Tobacco smoking Intervertebral disc degeneration Matrix proteoglycans Aggrecan Matrix metalloproteinases DNA damage repair |
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