不同PEG含量对mPEG-PLGA-mPEG纳米粒血浆蛋白吸附和体外细胞摄取的影响

目的考察不同PEG含量对mPEG-PLGA-mPEG(PELGE)纳米粒血浆蛋白吸附和巨噬细胞吞噬的影响。方法利用SDS-PAGE电泳技术,分析不同纳米粒的血浆蛋白吸附;以小鼠巨噬细胞RAW 264.7为模型,采用化学发光法分析不同纳米粒的吞噬情况。结果与结论PEG含量为5%~10%时,纳米粒吸附最少的血浆蛋白,而且被巨噬细胞吞噬也最少。由此,可设计出适合于静脉注射的、可生物降解的、长循环的药物载体。

Influence of different PEG contents on the phagocytic uptake and plasma protein adsorption of mPEG-PLGA-mPEG nanoparticles

OBJECTIVE To prepared monomethoxy(polyethylene glycol)-poly(D,L-lactic-co-glycolic acid)-monomethoxy(polyethylene glycol)(mPEGPLGE-mPET,PELGE) nanoparticles and study the influence of PEG content on the competitive plasma protein adsorption and particle uptake by RAW264.7.METHODS Plasma protein adsorption onto PELGE nanoparticles was analysed through SDS-PAGE electrophoresis.And chemiluminescence,which does not need labelling,was used to follow the the uptake of various PEGcoated nanoparticles with the phagocytic cells.RESULTS AND CONCLUSION A PEG content between 5%-10% was determined as a threshold value for optimal protein resistance and to avoid the uptake by RAW264.7 cells.When increasing the PEG content in the nanoparticles above 10% no further reduction was achieved.These results could be useful in the design of long circulating intravenously injectable biodegradable drug carries endowed with protein resistance properties and low phagocytic uptake.