罗丹明123介导的光动力学疗法预防急性移植物抗宿主病的实验研究

目的：探讨Rh123介导的光动力学疗法(PDT)预防异基因造血干细胞移植急性移植物抗宿主病(aGVHD)的可行性及安全性。 方法： 以C57B/6小鼠为供鼠，BALB/c小鼠为受鼠，建立小鼠异基因骨髓移植的aGVHD模型；混合脾脏淋巴细胞培养(MLC)加Rh123孵育，接受氩离子激光30 mW/cm2照射3 min，再与供者骨髓混合移植给受鼠，观察受鼠移植后造血重建、aGVHD发生情况及病理改变、生存率；流式细胞仪检测MLC细胞CD3+CD69+阳性率。 结果： 光动力学治疗组的aGVHD发生减少，肝、皮肤、肠道病理程度减轻，生存率显著高于未经光动力学治疗组；光动力学处理后的混合淋巴细胞培养24 h后，CD34+CD69+表达明显下降。 结论： Rh123介导的光动力学疗法可有效预防小鼠异基因骨髓移植的aGVHD。

The prevention of acute graft versus host disease by Rhodamine 123-mediated photodynamic therapy

AIM: To evaluate the effect and safety of Rhodamine 123 (Rh123)-mediated photodynamic treatment (PDT) on acute graft versus host disease. METHODS: An acute graft versus host disease (aGVHD) mice model was established using C57B/6 mice as donors and BALB/c mice as recipients. Mixed lymphocytic cells were cultured with Rh123 (50 nmol/L) and irradiated by argon laser 30 mW/cm2 for 3 min, then transplanted to BALB/c recipient mice mixed with donor bone marrow. Hepatopoietic recovery, aGVHD occurrence, survival time after transplantation and pathological changes were observed. In addition, CD3 CD69 positive rates of MLC were examined by flowcytometry. RESULTS: Occurrence of aGVHD decreased, degree of pathological manifestation became milder, survival rates were higher than non PDT groups. CD3 CD69 rates of MLC cells treated with photodynamic therapy (PDT) and cultured for 24 h significantly decreased. CONCLUSION: Rh123-mediated PDT can effectively prevent aGVHD of allogeneic bone marrow transplantation in mice.