向成骨细胞和成脂肪细胞分化的小鼠骨间充质干细胞调节单核细胞来源的破骨细胞发育

为探究分化阶段的骨间充质干细胞对于破骨细胞的影响,将小鼠密质骨来源的间充质干细胞体外诱导分化,研究其对于破骨细胞发育的调控作用。从小鼠密质骨中分离培养得到间充质干细胞,将其进行成骨和成脂肪诱导1周,分别将成骨分化和成脂肪分化的间充质干细胞与CD11b+的单核细胞共同培养,并以抗酒石酸的酸性磷酸酶染色鉴定破骨细胞。结果表明:向成骨分化和成脂肪分化的间充质干细胞均可以调节单核细胞向破骨细胞分化。与未分化的间充质干细胞相比,成骨分化的间充质干细胞促进破骨细胞发育的作用减弱,而成脂肪分化的间充质干细胞促进破骨细胞发育的作用增强。结论:间充质干细胞调节破骨细胞发育的能力在其分化为不同种类细胞后发生相应变化,提示间充质干细胞通过分化为子代细胞而对破骨细胞发育发挥选择性的调节。

Effect of Osteogenically and Adipogenically Differentiated Bone Mesenchymal Stem Cells from Mouse on Osteoclast Formation

This study was purposed to investigate the regulatory effects of differentiating mesenchymal stem cells（MSC） on osteoclast formation.The MSC from mouse compact bones were cultured and induced into osteoblasts and adipocytes for one week.To test their regulatory effect on osteoclastogenesis,osteogenically differentiated and adipogenically differentiated MSC were co-cultured with CD11b＋ monocytes and osteoclasts were identified with in situ tartrate-resistant acid phosphatase（TRAP） staining.The results showed that differentiated MSC supported osteoclastogenesis but the osteoclast supporting capacity of osteogenically differentiated MSC decreased as compared with undifferentiated MSC.More interestingly,the adipogenically differentiated MSC significantly promoted osteoclasts formation when co-cultured with monocytes.It is concluded that the regulatory effect of MSC on osteoclast formation has changed while they have differentiated into different types of cells.The findings indicate that MSC may exert alternative effect on osteoclastogenesis by differentiation to descendant cells.