获得性再生障碍性贫血患者外周血单个核细胞TRF1、TRF2、RAP1 mRNA表达研究

本研究检测获得性再生障碍性贫血患者外周血单个核细胞的TRF1、TRF2、RAP1基因表达水平,探讨其与获得性再生障碍性贫血发病的关系。采用实时荧光定量聚合酶链反应检测40例获得性再生障碍性贫血患者和20例正常对照组外周血单个核细胞中TRF1、TRF2、RAP1的基因表达情况。结果表明,获得性再生障碍性贫血患者的TRF1和RAP1 mRNA表达水平较正常对照组明显升高(P<0.05);TRF2 mRNA表达水平较正常对照组明显降低(P<0.01),并且TRF2与RAP1具有相关性(r=0.522,P=0.001)。结论:TRF1、TRF2、RAP1可能参与了获得性再生障碍性贫血的发病过程。

Expression of TRF1,TRF2 and RAP1mRNA in Peripheral Blood Mononuclear Cells of Patients with Acquired Aplastic Anemia

This study was aimed to investigate the expression levels of TRF1,TRF2 and RAP1 mRNA in peripheral blood mononuclear cells of patients with acquired aplastic anemia,and to explore their relation with onset of acquired aplastic anemia.Peripheral blood mononuclear cells of 40 patients with acquired aplastic anemia and 20 normal subjects as control were collected to detect mRNA expression of TRF1,TRF2 and RAP1 by using real-time quantitative polymerase chain reaction.The results showed that the expression levels of TRF1 and RAP1 in peripheral blood mononuclear cells of patients with acquired aplastic anemia were significantly higher than that in normal controls（P0.05）,while the expression level of TRF2 was lower than that in normal controls（P0.01）.There was significant correlation between TRF2 and RAP1 expressions level（r=0.522,P=0.001）.It is concluded that the changes in expression levels of TRF1,TRF2 and RAP1 may play a role in the pathogenesis of acquired aplastic anemia.