c-MPL在急性髓系白血病患者白血病干细胞中的表达

本研究通过检测c-MPL在急性髓系白血病(AML)患者骨髓细胞中的表达,探讨c-MPL表达与CD34、CD38的相关性,以确定c-MPL在白血病干细胞的表达。通过流式细胞术检测29例初诊AML患者的c-MPL和CD34、CD38阳性细胞比例;比较患者c-MPL表达水平与正常对照的差别及与临床指标的关系;分析患者的c-MPL表达水平与CD34、CD38各部分细胞分群的关系及它们之间的相关性。结果表明:AML患者骨髓细胞中c-MPL表达水平显著高于正常对照组(P<0.05);c-MPL的表达与患者的年龄、性别、白细胞计数、AML1-ETO融合基因、化疗后缓解情况无关;c-MPL在M2和M5分型中的表达显著高于正常对照组(P<0.05)。CD34阳性AML患者组的c-MPL表达水平显著高于CD34阴性AML患者组(P<0.01)。AML患者c-MPL在CD34+细胞中的表达显著高于CD34-细胞(P<0.001);AML患者中c-MPL在CD34+CD38+和CD34+CD38-两群细胞中的表达无显著性差异(P>0.05);c-MPL和CD34的表达呈正相关(r=0.380,P=0.042)。结论:c-MPL在AML患者中高表达,且与CD34的表达水平具有一定的正相关性,c-MPL表达于白血病干细胞。

Expression of c-MPL in Leukemic Stem Cells from Acute Myeloid Leukemia Patients

This study was aimed to investigate the expression of c-MPL in acute myeloid leukemia（AML） and the correlation of the c-MPL expression with CD34 and CD38,so as to define the expression of c-MPL in leukemic stem cells.The expression levels of CD34,CD38 and c-MPL were detected by flow cytometry in bone marrow cells from 29 newly diagnosed AML patients.The relationship of c-MPL positive cell ratio with clinical parameters and correlation of c-MPL with CD34 and CD38 expression in AML patients were analyzed.The results showed that expression level of c-MPL in AML patients was significantly higher than that of normal controls（P0.05）,and the expression level of c-MPL did not correlate with age,sex,white blood cell count,AML1-ETO fusion gene and remission after chemotherapy,but the expression of c-MPL in M2 and M5 patients was higher than that of normal control（P0.05）.Expression of c-MPL in CD34 positive AML patients was obviously higher than that in CD34 negative AML patients（P0.01）.c-MPL was significantly higher expressed in CD34＋ cells than that in CD34-cells（P0.001）,while c-MPL expression was not significantly different between CD34＋CD38-and CD34＋CD38-cell groups.Positive correlation between c-MPL and CD34 expression was observed（r=0.380,P=0.042）.It is concluded that expression of c-MPL is higher in AML patients,and positively correlates with the expression level of CD34.The c-MPL expresses in leukemic stem cells.