首页 | 本学科首页   官方微博 | 高级检索  
     


Emerging Phage Resistance in Pseudomonas aeruginosa PAO1 Is Accompanied by an Enhanced Heterogeneity and Reduced Virulence
Authors:Pawel Markwitz  Tomasz Olszak  Grzegorz Gula  Magdalena Kowalska  Michal Arabski  Zuzanna Drulis-Kawa
Affiliation:1.Department of Pathogen Biology and Immunology, University of Wroclaw, 51-148 Wroclaw, Poland; (P.M.); (T.O.); (G.G.);2.Division of Medical Biology, Institute of Biology, Jan Kochanowski University, 25-406 Kielce, Poland; (M.K.); (M.A.)
Abstract:Bacterial surface structures of a proteinic nature and glycoconjugates contribute to biofilm formation and provide shields to host defense mechanisms (e.g., the complement system and phagocytosis). A loss or alteration of these molecules, leading to phage resistance, could result in fewer virulent bacteria. In this study, we evaluate the biology and phenotype changes in Pseudomonas aeruginosa PAO1 phage-resistant clones, which emerge in phage-treated biofilms. We characterize these clones for phage-typing patterns, antibiotic resistance, biofilm formation, pathogenicity, and interactions with the innate immune system. Another important question that we address is whether phage-resistant mutants are also generated incidentally, despite the phage treatment-selective pressure, as the natural adaptation of the living biofilm population. It is found that the application of different phages targeting a particular receptor selects similar phage resistance patterns. Nevertheless, this results in a dramatic increase in the population heterogeneity, giving over a dozen phage-typing patterns, compared to one of the untreated PAO1 sessile forms. We also confirm the hypothesis that “phage-resistant bacteria are more susceptible to antibiotics and host-clearance mechanisms by the immune system”. These findings support phage application in therapy, although the overall statement that phage treatment selects the less virulent bacterial population should be further verified using a bigger collection of clinical strains.
Keywords:Pseudomonas aeruginosa   biofilm   phage-resistant mutants   virulence   heterogeneity
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号