冠状动脉粥样硬化组织环氧化物酶-2、诱导型前列腺素合成酶-1表达及塞来昔布的影响

目的探讨兔冠状动脉粥样硬化组织环氧化物酶-2(Cox-2)、诱导型前列腺素合成酶-1(mPGES-1)表达机制及塞来昔布对其表达的影响。方法采用32只雄性新西兰大白兔,正常对照组8只(A 组);另24只将给予1%高胆固醇喂养2周,建立兔动脉粥样硬化动物模型。将模型兔随机分为无干预组(B 组)、小剂量塞来昔布治疗组(15mg/kg,C 组),大剂量塞来昔布治疗组(35 mg/kg,D 组),每组8只。治疗4周后取双侧冠状动脉并分为平等2段,分别行半定量逆转录聚合酶链反应(RT-PCR)及蛋白印记法(Western blot)测定各组冠状动脉粥样硬化组织 Cox-2及 mPGES-1表达。结果与 A 组比较,B 组冠状动脉粥样硬化组织 Cox-2及 mPGES-1表达增高,差异具有统计学意义(P<0.05);与 B 组比较,C、D 组冠状动脉组织的 Cox-2及 mPGE-1表达明显下调,差异有统计学意义(P<0.05);与 B 组比较,C 组的 ALD 明显升高,差异有统计学意义(P<0.05)。结论在动脉粥样硬化的病理过程中炎症反应可能起着主导作用,塞来昔布干预可抑制 Cox-2及 mPGES-1表达,减缓动脉粥样硬化的进展。

Effects of Celecoxib on the expressions of Cox-2 and mPGES-1 in the rabbit coronary artery atherosclerotic tissue

Objective To explore the effects of Celecoxib on the expression of cyclooxygenase type 2(Cox-2)and membrane associated prostagladin E-1(mPGES-1)in the rabbit coronary artery atherosclerotic tissue.Methods Thirty-two male New Zealand white rabbits were randomly assigned into 4 groups:blank control group(group A), model group(group B),minidose Celecoxib-treated group(group C),large dose Celecoxib-treatedg roup(group D), rabbits in group B,C and D group were fed with 1% cholesterol diet for 2 weeks.Rabbits in groups C were subsequently treated with minidose Celecoxib(15mg/kg/day)and rabbits in group D with large dose Celecoxib (35mg/kg/day)for 4 weeks;Coronary arteries were excised after 4 weeks of treatment.The collected coronary arteries were equally dissected into 2 sections.Cox-2 and mPGES-1 expressions of coronary artery were evaluated by RT-PCR and western blot.Results There were significant down regulations of Cox-2 and mPGES-1 gene expressions(P<0.05)in group C and group D than in group B.Conclusions Celecoxib can downregulate Cox-2 and mPGES-1gene expressions,mitigate inflammation in atherosclerotic tissue,and slow down the process of atherosclerotie plaque formation.