宫颈癌组织中CDK1的表达及其与HPV_(16/18)感染的相关性

目的研究CDK1在宫颈癌组织中的表达及其与HPV16/18感染的相关性,以探讨宫颈癌的发生机制,进一步寻找有利于宫颈癌诊断的新的分子标志物,以指导其诊断与预后。方法采用免疫组织化学方法对50例宫颈癌石蜡标本、宫颈上皮内瘤样病变CI NⅠ20例、CI NⅡ~Ⅲ20例、正常宫颈组织20例进行CDK1的检测,同时采用PCR技术检测HPV16/18感染情况。结果①在宫颈癌组织中CDK1的阳性率高于CI N和正常宫颈组织,差异有统计学意义(P<0.05),且CDK1阳性表达率与病理分级有关,差异有统计学意义(P<0.05),CDK1阳性表达率与淋巴结转移、临床分期和年龄分组无关(P>0.05)。②HPV16/18在正常宫颈组织、CI N和宫颈癌中的阳性率逐渐升高,差异有统计学意义(P<0.05),但与年龄、临床分期、病理分级、淋巴结转移无关(P>0.05)。③CDK1与HPV16/18感染无关(rs=0.145,P>0.05)。结论宫颈上皮内瘤样病变及宫颈癌组织中CDK1表达的改变以及HPV16/18感染互相作用,共同影响CI N的发展及宫颈癌的发生。CDK1的异常表达与宫颈癌的恶变程度有关,可作为宫颈癌筛查、预后判断的有利指标。

Relationship between expression of CDK1 and HPV16/18 infection in uterine cervical carcinogenesis

Objective To investigate the relationship between expression of CDK1 and HPV16/18 infection in uterine cervical carcinogenesis.Methods Formalin-fixed,paraffin-embedded samples including 50 cases of squamous cell carcinomas,20 cases of cervical intraepithelial neoplasia CINⅡ～Ⅲ,20 cases of CINⅠ and 20 cases of normal cervical tissues were studied.Using Polymer detection system for immuno-histological staining(PV-9000) immunohisto-chemical technique,the authors examined the expression of CDK1 in these samples.The infections of HPV type 16,18 DNA were determined by PCR.Results ①There were significant differences for CDK1 among carcinomas,CIN and normal cervices(all P<0.05).CDK1 was correlated with tumor grades(all P<0.05).Expression of CDK1 was not correlated with lymph node metastasis,clinical stages and age group(all P>0.05).②The positive rate of HPV16/18 was of significant difference among cervical carcinomas,CIN and normal tissues(P<0.05) and was not associated with age group,tumor grades,clincal stages and lymph node metastasis(all P>0.05).③The expression of HPV16/18 was not associated with CDK1(rs=0.145,P>0.05).Conclusion The results suggest that in tissues of CIN and cervical carcinomas,changes in CDK1 expression and HPV16/18 infection interact with each other,which can influent the progression of CIN and carcinogenesis of cervical carcinomas.Expression of CDK1 is associated with uterine cervical carcinogenesis.It possibly becomes useful indexes for the primary screening and prognosis of cervical carcinomas.