肿瘤抗原MAGE—12的表位及二级结构预测

目的 预测肿瘤抗原MAGE－12的α－螺旋二级结构、B细胞表位和MAGE－12的HLA－A2限制性CTL表位。方法 用Garnier-Robson和Chou-Fasman方案预测肿瘤抗原MAGE－12的α－螺旋二级结构；用Kyte-Doolittle亲水方案预测肿瘤抗原MAGE－12的B细胞表位；用简单基序（simple motifs)和延展基序（extended motifs）对肿瘤抗原MAGE－12的HLA－A2限制性CTL表位进行预测。结果 MAGE－12可以含有较多的α－螺旋；B细胞识别的表位可能在82－93残基（QSDEGSSNEE－QE）或附近和3－14残基（LEQRSQHCKPEE）或附近；发现1个HLA－A2限制性CTL表位为FLWGPRALV（271－279）。结论 用亲水性方案和基序法预测肿瘤抗原MAGE－12的B细胞表位和MAGE－12的HLA－A2限制性CTL表位，为实验方法探索MAGE－12的表位提供有用线索。

Predication of secondary structures and epitopes of tumor antigen MAGE-12

ObjectiveTo predict the secondary structures, B-cell epitopes and the HLA-A2 restricted cytotoxic T lymphocyte (CTL) epitope of tumor antigen MAGE-12. Methods The secondary structures of tumor antigen MAGE-12 were predicted by Garnier-Robson's method and the antigenic epitopes of B cells were analysed by using hydrophilicity plot. HLA-A2 restricted CTL epitopes of MAGE-12 were predicted by the method of simple motif and extended motif. Results MAGE-12 are rich in alpha helix;the epitope recognized by B cells may be at 3-14 or adjacent to 82-93;and one HLA-A2 restricted CTL epitope of tumor antigen MAGE-12 is at 271-279(FLWGPRALV). ConclusionB-cell epitopes and CTL epitope of MAGE-12 were predicted by using hydrophilicity approach and motif plots. The predicted epitopes provided useful hints to probe epitopes in MAGE-12 by experiments.