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| 人脂肪干细胞向血管平滑肌细胞诱导分化的实验研究 | |
| 引用本文： | 蒋成,曹强,王继华,封兴华. 人脂肪干细胞向血管平滑肌细胞诱导分化的实验研究[J]. 中国美容医学, 2010, 19(2): 215-217 |
| 作者姓名： | 蒋成 曹强 王继华 封兴华 |
| 作者单位： | 1. 第四军医大学口腔医学院颌面整形外科,陕西,西安 2. 昆明医学院第二附属医院整形外科,云南,昆明,650101 |
| 摘 要： | 目的：体外诱导培养人脂肪干细胞（adi pose-der i ved st em cel l s,ADSCs）,观察是否可以分化形成血管平滑肌细胞（vascul ar smoot h muscl e cel l s,VSMCs）,为构建组织工程化血管寻找新的种子细胞来源。方法：免疫磁珠法分选收集原代脂肪干细胞,加入PDGF-BB、TGF-β1诱导14天后进行检测。结果：实验组细胞生长呈现血管平滑肌细胞所特有的峰谷样生长,血管平滑肌细胞特有的表面抗原标记物表达阳性。结论：脂肪干细胞定向诱导培养后,具有血管平滑肌细胞的特性,有可能成为构建组织工程化血管新的种子细胞来源。 |
| 关 键 词： | 脂肪干细胞 血管平滑肌细胞 血小板衍生生长因子 转化生长因子β1 |
Experiments of adscs induced into vascular smooth muscle cells |
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| JIANG Wei,CAO Qiang,WANG Ji-hua,FENG Xing-hua. Experiments of adscs induced into vascular smooth muscle cells[J]. Chinese Journal of Aesthetic Medicine, 2010, 19(2): 215-217 | |
| Authors: | JIANG Wei CAO Qiang WANG Ji-hua FENG Xing-hua |
| Affiliation: | 1.Department of Oral and Maxillofacial Surgery/a>;School of Stomatology/a>;Fourth Military Medical University/a>;Xi'an 710032/a>;Shaanxi/a>;China/a>;2. Department of Plastic Surgery/a>;the Second Affiliated Hospital of Kunming Medical College/a>;Kunming 650101/a>;Yunnan/a>;China |
| Abstract: | Objective To investigate the feasibility of ADSCs differentiating into VSMCs in vitro and to find the new source of seeding cells in constructing the tissue-engineered vessels. Methods To separate the cells from the original adipose stem cells by MACS and culture 14 days with PDGF-BB and TGF-β1. Results The cells show ＂peak and valley＂ growth pattern specified as mature VSMCs and express the VSMC＇s markers. Conclusion ADSCs were induced in vitro in specific environment,the cells show the significant characteristics of VSMCs and have the potentiality being seeding cells of construct the tissue-engineered vessels. |
| Keywords: | adipose-derived stem cells vascular smooth muscle cells platelet derived growth factor BB transforming growth factor beta 1 |
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