仙茅中酚苷类成分高效液相色谱法测定方法的建立

 目的 建立同时测定仙茅中多种酚苷类化合物的HPLC方法。方法 Angilent 1100 高效液相色谱仪，Zorbax SB C₁₈色谱柱；检测波长：230 nm；柱温：30 ℃；进样量 20 μL；流速1 mL·min^-1；流动相：乙腈(A)-0.1%醋酸水溶液(B)，梯度洗脱程序为：0~25 min， 4%~6%A； 25~58 min，6%~17%A；58~85 min，17%~22%A。结果 8种酚苷成分5-羟甲基糠醛(1)，2-羟基-5-(2-羟乙基)苯基-β-D-吡喃葡萄糖苷(2)，苔黑酚龙胆二糖苷(3)，苔黑酚葡萄糖苷(4)，苔黑酚-1-O-β-D-呋喃芹糖基-(1→6)-β-D-吡喃葡萄糖苷(5)，2， 6-二甲氧基苯甲酸(6)，仙茅苷(7)和仙茅素A(8) 的质量浓度和峰面积呈良好的线性关系，平均加样回收率符合含量测定要求。结论 该方法简便、灵敏、重复性好，适用于同时测定仙茅中多种酚苷类成分。

Simultaneous Determination of Phenolic Glycosides in Curculigins Rhizoma by HPLC

OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of phenolic glycosides in Curculigins Rhizoma.METHODS Angilent 1100 HPLC on a ZORBAX SB C18 column(4.6 mm×250 mm,5 μm) with Extend C18 guard column(4.6 mm×12.5 mm,5 μm) were used.The mobile phase consisted of acetonitrile(A)-0.1% phosphoric acid aqueous(B).The gradient elution program was as follows: 0-25 min,4%-6%A;25-58 min,6%-17%A;58-85 min,17%-22%A.The flow rate was kept at 1 mL·min-1,and the column temperature was set at 30 ℃.The detection wavelength was 230 nm.RESULTS The concentrations and peak areas of the 8 phenolic glycosides including 5-hydroxymethylfurfural(1),2-hydroxy-5-(2-hydroxyethyl)phenyl-β-D-glucopyranoside(2),anacardoside(3),orcinol glucoside(4),orcinol-1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(5),2,6-dimethoxybenzoic acid(6),curculigoside(7) and curculigine A(8) were in good linear relationship.The average recoveries of the 8 constituents met the requirement for determination.CONCLUSION The method is simple,sensitive,reproductive,and suitable for simultaneous determination of a variety of phenolic glycosides in Curculigins Rhizoma.