肉毒碱复合高脂饲料喂养兔血脂水平的变化

背景：肉毒碱是脂肪酸进入线粒体内进行氧化代谢的惟一转运载体，其在机体内水平对血脂代谢具有重要的影响作用。 目的：观察肉毒碱对高脂饲料喂养兔血脂水平变化的影响。 设计、时间及地点：随机对照动物实验，于2006-10/2007-05在解放军南京军区南京总医院检验科及比较医学科完成。 材料：普通级健康纯系雄性新西兰兔14只，体质量(1.98±0.22) kg，用于建立兔高脂血症模型。 方法：14只雄性新西兰兔随机抽签法分为对照组及实验组，每组7只。对照组仅用高脂饲料喂养，实验组用高脂饲料喂养的同时每天补充肉毒碱200 mg/kg 体质量。分别于实验开始后第0，2，3，4周称体质量并抽取耳动脉血，分离血清后测定血中总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇以及载脂蛋白B和血清游离肉毒碱水平。 主要观察指标：不同时间点各组动物体质量的变化以及血中总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇以及载脂蛋白B和血清游离肉毒碱水平。 结果：14只新西兰兔全部进入结果分析。4周内，实验组和对照组体质量差异无显著性意义(P > 0.05)。自第2周开始，实验组兔血清游离肉毒碱水平高于对照组(P < 0.01)；总胆固醇、载脂蛋白B水平明显低于对照组(P < 0.05或P < 0.01)；3周后，实验组血清三酰甘油、低密度脂蛋白胆固醇水平也低于对照组(P < 0.01)；两组高密度脂蛋白胆固醇水平差异无显著性意义(P > 0.05)。 结论：补充服用肉毒碱能够抑制高脂饮食导致的血中总胆固醇、三酰甘油、低密度脂蛋白胆固醇以及载脂蛋白B水平升高。

Changes of serum lipid level in rabbits fed with carnitine combined with high-fat diet

BACKGROUND: Carnitine, which is the only carrier for the fatty acid crossing into mitochondria for oxidize, has important effect on serum lipids metabolism. OBJECTIVE: To investigate the effect of carnitine supplement on the level changes of serum lipids in rabbits fed with high-fat diet. DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at the Department of Clinical Laboratory, General Hospital of Nanjing Military Area Command of Chinese PLA from October 2006 to May 2007. MATERIALS: 14 male New Zealand rabbits, weighting (1.98±0.22) kg, were adopted to establish hyperlipidemia model. METHODS: 14 rabbits were randomly divided into experimental and control group, with 7 animals in each. Rabbits in the control group were fed with high-fat diet, and those in the experimental group were supplied with 200 mg/kg carnitine everyday combined with high-fat diet. The blood was collected from central artery and body weight was obtained at 0, 2, 3, 4 week after the experiment, then total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, apolipoprotein B and free carnitine levels in serum were measured. MAIN OUTCOME MEASURES: Changes of body wight, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, apolipoprotein B and free carnitine levels in serum were measured at various time points in each group. RESULTS: All 14 rabbits were involved in the result analysis. Body weight of the rabbits between the experimental group and control group showed no difference in 4 weeks (P > 0.05). From the second week, serum carnitine level in the experiment group was higher than that of the control group (P < 0.01), total cholesterol and apolipoprotein B levels were significant lower than those in the control group (P < 0.05 or P < 0.01). After the third week, the levels of triglyceride and low density lipoprotein cholesterol in the experiment group were lower than those in the control group (P < 0.01). There was no significant difference of high density lipoprotein cholesterol level between the two groups (P > 0.05). CONCLUSION: Carnitine supplement can inhibit the increasing of total cholesterol, triglyceride, low density lipoprotein cholesterol and apolipoprotein B levels in serum of the rabbits fed with a high-fat diet.