| Stathmin和CrkL蛋白在耐阿霉素的K562白血病细胞中表达上调 |
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| 作者姓名： | Xu JP Hu JD Lin MH Li J Liu TB |
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| 作者单位： | 1. 福建医科大学附属协和医院血液病研究所、福建省血液病学重点实验室,福建福州350001;福建医科大学医学技术与工程学院检验系,福建福州350004
2. 福建医科大学附属协和医院血液病研究所、福建省血液病学重点实验室,福建福州,350001 |
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| 基金项目： | 国家自然科学基金(编号30572132); 福建省自然科学基金(编号C0410029); 福建医科大学基金(编号FJGXY04022) |
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| 摘 要： | 本研究应用蛋白质组学方法比较白血病K562细胞及其耐阿霉素的K562/A02细胞中蛋白质表达谱的差异,筛选新的耐药相关蛋白。利用荧光差异双向电泳（2D-DIGE）技术分离K562细胞及K562/A02细胞的总蛋白,用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/MS）对差异表达蛋白质点进行鉴定,搜索蛋白质数据库,获得差异蛋白质的信息,并用蛋白免疫印迹法和实时荧光定量PCR法在蛋白质水平和mRNA水平对差异蛋白质进行验证。结果显示,经过DIGE技术和质谱分析,鉴定出8个蛋白质在耐阿霉素的K562细胞中表达有差异,其中2个表达下调,6个表达上调,它们分别参与细胞能量代谢、细胞增殖、细胞凋亡、细胞信号转导和基因转录等。在表达上调的蛋白中挑选Stathmin和CrkL蛋白,在K562及其耐药细胞株中进行了蛋白免疫印迹实验,结果与双向电泳结果一致;实时荧光定量PCR显示,CrkL在mRNA水平变化与在蛋白质水平变化一致,而Stathmin在mRNA水平变化与蛋白质水平变化不完全一致。结论：白血病K562细胞及其耐阿霉素K562/A02细胞蛋白质表达谱有差异,Stathmin和CrkL蛋白可能与耐药有关,值得进一步探讨。
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| 关 键 词： | Stathmin蛋白 CrkL蛋白 阿霉素 多药耐药 K562细胞 K562/A02细胞 |
Up-regulation of Stathmin and CrkL protein expressions in adriamycin-resistant leukemia cell line K562/A02 |
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| Xu JP,Hu JD,Lin MH,Li J,Liu TB.Up-regulation of Stathmin and CrkL protein expressions in adriamycin-resistant leukemia cell line K562/A02[J].Journal of Experimental Hematology,2011,19(6):1383-1387. |
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| Authors: | Xu Jian-Ping Hu Jian-Da Lin Min-Hui Li Jing Liu Ting-Bo |
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| Institution: | XU Jian-Ping1,2,HU Jian-Da1,LIN Min-Hui1,LI Jing1,LIU Ting-Bo1 1Fujian Provincial Key Laboratory of Hematology,Fujian Institute of Hematology,Fujian Medical University Union Hospital,Fuzhou 350001,Fujian Province,China,2Department of Laboratory Examination,College of Medical Technology and Engineering,Fujian Medical University,Fuzhou 350004 |
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| Abstract: | The purpose of this study was to compare the differences of the protein expression profiles between human myeloid leukemia K562 cells and adriamycin-resistant K562/A02 cells,as well as to select novel resistance-related proteins in myeloid leukemia by means of proteomics.The total cellular proteins were separated from K562 and adriamycin-resistant K562/A02 cells by using technigue of two dimensional difference in gel electrophoresis(2D-DIGE).Differentially expressed proteins were analyzed by matrix-assisted... |
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| Keywords: | Stathmin protein CrkL protein adriamycin multi-drug resistance K562 cell K562/A02 cell |
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