冰茶栓对骨癌痛大鼠脊髓GFAP及其mRNA表达的影响

目的观察冰茶栓对W256细胞诱导的骨癌痛大鼠脊髓GFAP及其mRNA表达的影响,以探讨其抗骨癌痛的中枢机制。方法将SD大鼠分成假手术组、模型组、给药组三组。给药组给予冰茶栓101mg?kg-1;给药10d后截取脊髓组织,检测GFAP蛋白及mRNA表达。结果给药组大鼠脊髓组织GFAP蛋白及其mRNA表达均较模型组显著降低(P<0.01;P<0.05)。结论冰茶栓抗骨癌痛作用的中枢机制可能与其抑制星形胶质细胞的激活有关。

Effects of Bingcha Embolus on GFAP Production and Its mRNA Expression in Spinal Cord in Rat Model of Bone Cancer Pain

Objective To observe the effects of Bingcha Embolus on GFAP production and its mRNA expression in spinal cord in the rat model of bone cancer pain induced by W256 tumor cells. Methods Sprague-Dawley female rats were divided into three groups: sham group, model group and Bingcha Embolus group. After 15 days, rats in the sham group and in the model group were treated with blank embolus, and rats in Bingcha Embolus group were treated with Bingcha Embolus (101 mg-kg-1). After 10 days’ treatment, the spinal cord tissues of the three groups were taken out to examine the changes in protein production and mRNA expression of GFAP by immunohistochemistry and RT-PCR respectively. Results The protein production and mRNA expression of GFAP in the spinal cord in Bingcha Embolus group have decreased remarkably comparing with that of the model group(P <0.01 ;P <0.05). Conclusion Bingcha Embolus’s analgesic mechanism in bone cancer pain is probably because of its inhibition of astrocytes’ activeness.