单核细胞趋化蛋白-1表达与狼疮肾炎

目的探讨单核细胞趋化蛋白-1(MCP-1)在狼疮肾损伤中的作用.方法应用ELISA方法测定112例系统性红斑狼疮(SLE)患者、30例类风湿性关节炎患者、11例非SLE肾损伤患者及40例正常健康者血清MCP-1水平;应用RT-PCR方法,测定以上各组外周血单个核细胞(PBMCs)MCP-1 mRNA表达.结果狼疮处于疾病活动时PBMCs MCP-1 mRNA表达上调,尤其是狼疮肾炎活动组患者PBMCs MCP-1 mRNA表达增加最为显著(P＜0.001),PBMCs MCP-1 mRNA表达不仅与总狼疮疾病活动指数评分呈正相关(r=0.6245,P＜0.001),而且与狼疮疾病活动指数肾评分呈正相关(r=0.6808,P＜0.001),而在类风湿性关节炎组和非狼疮肾脏疾病组仅低水平表达,与正常对照组水平相近似(P＞0.05).狼疮活动组血清MCP-1水平较非活动组及正常对照组均明显升高(P＜0.001),但活动性狼疮肾炎组与无肾损伤组之间血清MCP-1水平的差异无显著性(P＞0.05).结论狼疮处于疾病活动时PBMCs MCP-1 mRNA表达上调,并与狼疮肾炎的疾病活动情况密切相关.

Expression of monocyte chemoattractant protein-1 and lupus nephritis

Objective To explore the role of monocyte chemoattractant protein-1(MCP-1)in lupus nephritis(LN). Methods Sera MCP-1 levels were measured by enzyme linked immunosorbent assay in 112 patients with systemic lupus erythematosus(SLE), 30 patients with rheumatoid arthritis, 11 non-SLE patients with renal impairment, and 40 healthy volunteers. MCP-1 mRNA expression in peripheral blood mononuclear cells(PBMCs)was also investigated with reverse trancription-polymerase chain reaction semi-quantitative method. Results The expression of MCP-1 was significantly higher in active LN groups than in all other groups(P < 0.001), and there was a close correlation between MCP-1 expression and the overall SLE disease activity index score(r = 0.6245, P < 0.001)and the SLE disease activity index renal score(r = 0.6808, P < 0.001). Low expression of MCP-1 was observed in diseased controls and healthy controls. The sera levels of MCP-1 were significantly higher in patients with active diseases than in patients with inactive SLE and controls, but no significant difference were found between the active LN groups and non-renal involvement group(P >0.05). Conclusion The expression of PBMCs MCP-1 mRNA is upregulated in active SLE. Meanwhile, its expression levels are correlated with the activity of LN.