ZNF262在常染色体显性多囊肾病患者肾组织中的表达及意义

目的:检测锌指蛋白262(ZNF262)在正常肾组织及不同发病阶段多囊肾组织中的表达,并以增殖细胞核抗原(PCNA)为对照,初步探讨ZNF262在常染色体显性多囊肾病(autosomal dominant polycystic kidney disease,ADPKD)中的作用.方法:根据肾小球滤过率(GFR)大小对ADPKD(n=8)进行分期,观察其影像学、常规病理检查结果,采用半定量RTPCR方法检测人正常肾组织(n=8)、早期多囊肾组织(n=4)、晚期多囊肾组织(n=4)中ZNF262和PCNA的表达,并对上述组织中ZNF262和PCNA的表达进行相关性分析.结果:与正常肾组织相比,ZNF262和PCNA在早期、晚期多囊肾组织中的表达均显著增多(P＜0.01),且晚期多囊肾组织表达高于早期多囊肾组织(P＜0.05).ZNF262和PCNA在早期、晚期多囊肾及正常肾组织中的表达存在显著的相关性(r1=0.842 6,r2=0.902 1,r3=0.883 5,均P＜0.05).结论:与正常组织相比,ZNF262不仅在ADPKD高表达,并且随着病程进展表达逐步增高,这与PCNA的表达具有显著的相关性.提示ZNF262可以作为检测ADPKD的指标,并有助于临床进展分期的判别.

Expression of zinc finger protein 262 mRNA in patients with autosomal-dominant polycystic kidney disease and its significance

Objective:To investigate the expression of zinc finger protein 262(ZNF262) mRNA in normal kidney tissues and kidney tissues of patients with autosomal-dominant polycystic kidney disease(ADPKD) at different stages,and to explore the role of ZNF262 in pathogenesis of ADPKD.Methods: Patients with ADPKD were staged according to glomerular filter rate(GFR).Imaging observation and routine pathological examination were performed. The expression of ZNF262 mRNA and proliferating cell nuclear antigen(PCNA) mRNA in normal kidney tissues(n=8),early stage ADPKD kidney tissues(n=4) and advanced stage ADPKD kidney tissues(n=4) was examined by semi-quantitative RT-PCR.The correlation between the expressions of the 2 genes was investigated in all tissue specimens.Results: Expression of ZNF262 mRNA and PCNA mRNA in early and advanced ADPKD kidney tissues was significantly higher than that in normal renal tissues(both P<0.01),and that in the advanced stage ADPKD was significantly higher than that in early stage ADPKD(both P<0.05).The expression of ZNF262 and PCNA mRNA was highly correlated in the early,advanced ADPKD and normal renal tissues(r_1=0.842 6,r_2=0.902 1 and r_3=0.883 5,respectively,all P<0.05).Conclusion: The ZNF262 mRNA level is higher in ADPKD kidney tissue than that in normal control and increases with the advancement of ADPKD.The expression of ZNF262 is significantly correlated with the expression of PCNA in the same renal tissues.The expression of ZNF262 mRNA may serve as an indicator in diagnosis of ADPKD and may be used for clinical staging of ADPKD patients.