用McAb-AST及骨髓涂片法对四川省汶川县犬感染利什曼原虫的调查研究

作者等用单克隆抗体-抗原斑点试验(McAb-AST)及骨髓穿刺涂片法对汶川县125只犬进行感染利什曼原虫调查,骨髓涂片法查出原虫的阳性犬46只,感染率为36.8％,较之历年来陇南、川北报道的犬感染率为高,不但查明了当地犬感染利什曼原虫的严重性,且为本年在白蛉繁殖季节前消除这些传染源提供了确切依据。为了研究对犬利什曼病的简易、准确的调查方法,我们同时用McAb-AST对该125只犬的血清,检测其循环抗原,结果与骨髓涂片阳性符合率为97.8％,总符合率95.2％,可望取代骨髓涂片法。

A Survey of Dog Infected with Leishmania Donovani in Wen-chuan County of Sichuan Province by McAb-AST and Bone-Marrow Smear Methods

In the present study, the amastigotes of Leishmania were found on the bone marrow smears of 46 out of 125 dogs examined in Wen-chuan county with a positive rate of 36. 8% , which is the highest among all the records which have been reported from South of Gansu and north of Sichuan up to now. The severity of the canine leishmaniasis was declared by this study. In order to develop a simple method for the survey in this field,we used McAb-AST to detect the circulating antigen of Leishmania with a result of 97. 8% positive rate of coincidence and 95. 2% total rate of coincidence comparing with bone marrow smear method. It is suggested that McAb-AST will be a promising technique instead of bone marrow smear method.