不同品系小鼠胚胎玻璃化冷冻保存的比较研究

目的　研究甘油作为冷冻保护剂、不同基因型小鼠对胚胎玻璃化冷冻的影响。方法　采用 6 5mol L的甘油作为冷冻保护剂 ,采用二步法对CBA、NOD、C57BL 6J、ICR及CD1小鼠 3 5d的胚胎进行玻璃化冷冻 ,并比较了不同品系小鼠胚胎的复苏率及移植受孕率。结果和结论　CBA、NOD、C57BL 6J,ICR及CD1的复苏率分别为 5 7 6 %、4 8%、31 3%、86 5 %及 88% ,移植受孕率为 2 1%、2 3 5 %、11%、38%和 35 5 % ,封闭群小鼠的胚胎复苏率、移植受孕率均显著高于近交系小鼠。这提示胚胎的复苏率及移植受孕率可能与小鼠的不同基因型有关。五个品系中 ,桑椹胚及早期囊胚的体外复苏率均显著高于扩张囊胚。这说明不同基因型及胚胎的不同发育阶段对胚胎玻璃化冷冻效果有影响

Studying the Different Strains of Mouse Embryo Cryopreservation in a Vitrification Solution

Objective In order to study the effect of the genotype of different strains on mouse embryo cryopreservation in the glycerol vitrification solution.Methods The 6 5mol/L glycerol and 6% (w/v) BSA were used as the vitrification solution for the cryopreservation of morulae and blastocysts embryos of CBA,NOD,C 57 BL/6J,ICR and CD1 mouse.Results and Conclusion The rates of in vitro development of CBA,NOD,C 57 BL/6J,ICR and CD1 mouse embryos to expanded blastocysts ranged from 31 3% to 88% after vitrification.The rates of CBA,NOD,C 57 BL/6J,ICR and CD1 mouse embryos to normal fetuses was 21%,23 5%,11%,38%,35 5%.The rates of in vitro development of CBA,NOD,C 57 BL/6J6,ICR and CD1 mouse morulae and early blastocysts were significantly higher than expanded blastocystst.The in vitro development and in vivo development were significantly influenced by inbred strain,closed colony and different stages of mouse embryo.The result of mouse embryo cryopreservation in the vitrification solution may be connected with the different genotype of the different strains and different stages of mouse embryo.