碱性成纤维细胞生长因子对大鼠晚期周围神经再生作用的实验研究

目的探讨外源性碱性成纤维细胞生长因子(bFGF)对晚期周围神经再生的作用.方法50只SD大鼠随机分治疗组、对照组各25只,切断右侧坐骨神经,12周后予以修复,修复术后每日分别给予bFGF和生理盐水,行神经电生理和组织学检查.结果治疗组和对照组修复处远段神经均有不同程度再生,4周时已可见到再生轴突,且治疗组多见.计量分析治疗组运动神经传导速度、神经肌肉动作电位幅值、髓鞘厚度、再生轴突直径和截面积明显优于对照组.治疗组与对照组相比,差异有显著性.结论bFGF能促进晚期周围神经再生.

Experimental Study of Basic Fibroblast Growth Factor on Delayed Peripheral Nerve Regeneration in Rats

Objective To study the effect of exogenous basic fibroblast growth factor (bFGF) on delayed peripheral nerve regeneration. Method Transection was made on the right sciatic nerve of 50 Sprague Dawley rats. Reparing operation was made 12 weeks after transection. bFGF was injected into 25 rats as experimental group and only saline into other 25 rats as control group.All animals were observed through neuroelectro physiological examinations and histological examinations at 2,4,6,8 and 12 weeks after the reparing operation. Results In both experiment and control groups, there could be found significant nerve regeneration. In experiment group, more axons grew in the distal nerve segment at the fourth week postoperatively. The motor nerve conductive velocity,the muscle evoked action potential, the thickness of myelin, and the diameter and cross section area of regenerating axons in experimental group were better than those in the control group with a great difference. Conclusion bFGF can enhance the delayed peripheral nerve regneration.