脑心康片对大鼠脑缺血再灌注损伤后凋亡相关基因Caspase-3表达的影响

[目的]观察脑心康片对大鼠脑缺血再灌注损伤的保护作用,进一步探讨其作用机制。[方法]Wistar大鼠雄性70只,按照体质量随机分为7组,每组10只。即脑心康片高剂量组、中剂量组、低剂量组、尼莫地平组、银杏叶片组、模型组和假手术组,分别给等体积生理盐水,各组均连续灌胃给药7d。末次给药30min后,采用大鼠大脑中动脉线栓法制备不完全脑缺血模型,观察脑心康片给药前后大脑中动脉阻塞(MCAO)大鼠神经功能缺损导致的行为学变化;免疫组织化学法检测脑心康片对脑缺血后24、48、72h脑组织半胱氨酰天冬氨酸激酶-3(Caspase-3)蛋白表达的影响。[结果]脑心康片能明显改善脑缺血大鼠的神经功能缺损症状,抑制Caspase-3蛋白的表达。[结论]脑心康片对脑缺血具有保护作用,遵循一定的时效关系和量效关系,其机制可能与脑心康片抑制凋亡相关基因的表达,进而抑制神经元细胞凋亡有关。

Effect of Naoxinkang tablet on apoptosis-related gene Caspase-3 expression in rats with cerebral ischemia reperfusion injury

[Objective] To observe the protective effect of Naoxinkang(improving the function of brain and heart) tablet on cerebral ischemia reperfusion injury in rats and furthermore explore the effect mechanism in treating cerebral ischemia in rats. [Methods] Wistarmale rats were randomly divided into seven groups:the high,middle,low dosage of Naoxinkang group,Nimodipine group,ginkgo leaf tablet group,model group and sham-operated group. The incomplete cerebral occlusion of middle cerebral artery in rats was induced with fishing line(MCAO). The effect of Naoxinkang tablet on protein expression of cysteiny aspartate specific protease-3(Caspase-3) was determined with immunohistochemical process at 24h,48h and 72h after cerebral ischemia,and the effect of Naoxinkang tablet on the change of ethology and pathomorphology due to nerve injury of rats with MCAO before and after treatment were also observed. [Results] Naoxinkang tablet could obviously improve pathomorphology of cerebral ischemia,inhibit the expression Caspase-3. The best therapeutic effect was observed in high dose group of Naoxinkang tablet,and the better therapeutic effect was also got with longer treatment course. [Conclusion] Naoxinkang tablet has certain time-effect and dose-effect relationship in protecting effect on cerebral ischemia. The mechanism of Naoxinkang tablet against cerebral ischemia is related to anti-inflammatory action,inhibition of cell apoptosis and affecting the related gene expression interrelated with cell apoptosis.