白芍与柴胡不同比例配伍白芍总苷及苯甲酸水煎出量的比较

目的]考察白芍与柴胡不同比例配伍共煎芍药总苷及苯甲酸的煎出量,以探讨传统药对白芍配伍柴胡对有效成分及有害成分煎出的影响。方法]单味白芍、白芍与柴胡配伍(1:2,1:1,2:1),水煎提取,分别直接分离和水解后再分离测定游离苯甲酸及芍药总苷,采用高效液相色谱法(HPLC)测定。结果]白芍配伍柴胡,游离苯甲酸煎出量均比白芍单煎明显降低,芍药总苷煎出量与白芍单煎比变化不大。结论]白芍配伍柴胡合煎与白芍单煎相比,可显著降低有害成分苯甲酸的煎出量。

Comparison of water decocted quantity of total glycosides of Radix Paeoniae ALba and benzoic acid with different compatibility of Radix Paeoniae ALba and Radix Bupleuri

Objective] To observe the decocted quantity of the total glycosides of Radix Paeoniae ALba and benzoic acid when co-decocting with different compatibility of Radix Paeoniae Alba and Radix Bupleuri and to explore the influence the traditional compatibility of Radix Paeoniae Alba and Radix Bupleuri on the decocted quantity of the effective and harmful constituent.Methods] Single Radix Paeoniae Alba,Radix Paeoniae Alba concomitant with Radix Bupleuri(1:1,1:2,2:1) were boiled with water.Direct isolation or isolation after being hydrolyzed were performed to determining the isolated benzoic acid and the total glycoside of the Radix Paeoniae Alba respectively with HPLC.Results] The isolated quantity of benzoic acid was more decreased when co-decocting Radix Peaoniae Alba with Radix Bupleuri than single Radix Paeoniae Alba was decocted,however the extracted quantity of the total glycoside was not different.Conclusion] The extracted quantity of benzoic acid,a harmful constituent,can be lowered significantly when Radix Paeoniae Alba was co-decocted with Radix Bupleuri than single Radix Paeoniae Alba was decocted.