Genotoxic and hematological effects of chlorpyrifos exposure on freshwater fish Labeo rohita |
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| Authors: | Muhammad Ismail Rahat Ali Muhammad Shahid Muhammad Asaf Khan Muhammad Zubair Tayyaba Ali |
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| Affiliation: | 1. Environmental Toxicology Laboratory, Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan,;2. Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan,;3. Environmental Toxicology Laboratory, Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan,;4. Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan,;5. Department of Zoology, Government College University, Faisalabad, Pakistan |
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| Abstract: | ![]()
Chlorpyrifos is a commonly used organophosphate insecticide that causes toxicological effects in aquatic organisms especially in fish. This study determined the effects of chlorpyrifos on the genotoxic and hematological parameters of freshwater fish, Labeo rohita. The genotoxic effects of different sublethal concentrations of chlorpyrifos were investigated in the erythrocytes of Labeo rohita (commonly known as Rohu) using the Micronucleus test. Effects of chlorpyrifos on the hematological parameters of the fish were also observed. Fish specimens were exposed to three sublethal concentrations of chlorpyrifos viz., sublethal I (SL-I, 1/6th of LC50?=?～73.8?μg/L), sublethal II (SL-II, 1/4th of LC50?=?～110.7?μg/L) and sublethal III (SL-III, 1/2nd of LC50?=?～221.4?μg/L) for 96?h. Blood samples were collected at every 24?h and were subjected to the Micronucleus assay. The observed micronucleus frequencies were concentration and time-dependent. The MN induction was significantly highest (p?
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| Keywords: | Chlorpyrifos micronucleus test hematology Labeo rohita toxicity |
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