Discovery of a dsRNA virus infecting the marine photosynthetic protist Micromonas pusilla |
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Authors: | Brussaard C P D Noordeloos A A M Sandaa R-A Heldal M Bratbak G |
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Institution: | Department of Biological Oceanography, Royal Netherlands Institute for Sea Research, NL-1790 AB Den Burg, The Netherlands. corina.brussaard@nioz.nl |
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Abstract: | We report the isolation of the first double-stranded (ds) RNA virus in the family Reoviridae that infects a protist (microalga Micromonas pusilla, Prasinophyceae). The dsRNA genome was composed of 11 segments ranging between 0.8 and 5.8 kb, with a total size of approximately 25.5 kb. The virus (MpRNAV-01B) could not be assigned to the genus level because host type, genome size, and number of segments smaller than 2 kb did not correspond to either of the two existing 11-segmented dsRNA genera Rotavirus and Aquareovirus. MpRNAV-01B has a particle size of 65-80 nm, a narrow host range, a latent period of 36 h, and contains five major proteins (120, 95, 67, 53, and 32 kDa). MpRNAV-01B was stable to freeze-thawing, resistant to chloroform, ether, nonionic detergents, chelating and reducing agents. The virus was inactivated at temperatures above 35 degrees C and by ionic detergent, ethanol, acetone, and acidic conditions (pH 2-5). |
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Keywords: | dsRNA virus 11-Segmented genome Micromonas pusilla Phytoplankton Protist Reoviridae |
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