CD34~+细胞含量对自体骨髓干细胞移植治疗肝硬化疗效的影响

目的研究分离骨髓干细胞后CD34+细胞含量对自体骨髓干细胞移植治疗肝硬化疗效的影响。方法回顾性分析自体骨髓干细胞移植治疗肝硬化病例41例,其中CD34+细胞含量不足5%的27例,CD34+细胞含量超过5%(含5%)的14例。分析上述病例术后2～4周的实验室检测指标。结果在CD34+细胞含量不足5%的病例中,患者术后的血清总蛋白、白蛋白(Alb)、碱性磷酸酶(ALP)和血小板(PLT)均较术前明显增高(P<0.05)。在CD34+细胞含量超过5%的病例中,患者术后的血清总蛋白、Alb较术前明显增高(P<0.05)。术前CD34+细胞含量超过5%病例组的PLT平均值(88.357±38.454)×109/L]明显低于CD34+细胞含量不足5%的病例组(132.590±127.260)×109/L]。结论自体骨髓干细胞移植可以有效的提升肝硬化患者血清总蛋白和Alb水平,本组病例中CD34+细胞含量的不同并未明显影响术后患者血清总蛋白、Alb的恢复水平。自体骨髓干细胞移植可能影响胆汁的排泌。两组病例脾功能亢进程度不同可能是导致患者CD34+细胞含量差异的原因。

The effects of CD34~+ cell on the efficacy of autologous bone marrow stem cell transplantation in patients with cirrhosis

Objective To investigate the effects of CD34+ cell on the efficacy of autologous bone marrow stem cell transplantation in patients with cirrhosis.Methods Forty one patients with cirrhosis treated with autologous bone marrow stem cell transplantation were analyzed retrospectively.There were 27 cases with CD34+ cell less than 5% and 14 cases with CD34+ more than 5%(including 5%).The results of laboratory test of the patients during 2-4 weeks after the transplantation were collected and analyzed.Results After transplantation,the level of serum total protein,albumin,ALP and platelet of patients with CD34+ less than 5% was higher than that of pretransplantation(P<0.05) and the level of serum total protein and albumin of patients with CD34+ more than 5% was higher than that of pretransplantation(P<0.05).Before transplantation,the platelet count of patients with CD34+ more than 5%(88.3 571±38.45 398×106/L)was lower than that of patients with CD34+ less than 5%(132.5 900±127.25 966×106/L).Conclusion Autologous bone marrow stem cell transplantation could improve the level of serum total protein and albumin of patients with cirrhosis and CD34+ cell count didn’t affect the improvement of serum total protein and albumin after transplantation.Autologous bone marrow stem cell transplantation may have effect on the bile drainage.Different degree of hypersplenia may be the reason of patients having different CD34+ cell count.