Sysmex XE-2100全自动血细胞分析仪对血细胞形态异常警示功能的评价和分析

目的探讨Sysmex XE-2100全自动血细胞分析仪对形态异常细胞提示功能的评价。方法对855例各种住院本院患者标本,进行血涂片复检,评价其对异常细胞报警系统的性能。结果仪器提示血小板减少与血涂片显微镜镜检结果相比较Kappa值0.97,U=16.90,差异有统计学意义(P<0.01),显示两者一致性满意;提示核左移的仪器结果与镜检结果比较Kappa值0.68,U=9.43,差异有统计学意义(P<0.01),提示两者一致性尚可;而提示未成熟粒细胞、异型淋巴细胞、原始粒细胞、有核红细胞、血小板聚集的仪器结果与镜检结果比较,Kappa值分别为0.35、0.31、0.27、0.28、0.25,U值分别为7.15、4.89、4.66、4.17、4.55,差异有统计学意义(P<0.01),显示两者一致性尚不够理想。依据标准复检血涂片,敏感度为95.70%,特异度为81.16%,准确性为85.67%。结论Sysmex XE-2100血液分析仪的报警信息提示只起到一个筛选及提示的作用,可以降低劳动强度,提高工作效率,但是并不能完全代替显微镜检查,必须学会综合分析各种干扰因素,给临床提供全面、准确可靠的检验报告。

Evaluation and analysis functions of Sysmex XE-2100 automated blood cell analyzer for warning abnormal blood cell morphology

Objective To appraise the warning function of the Sysmex XE-2100 analyzer for abnormal cells. Methods 855 samples were examined repeatedly by microscope measurement for researching it＇s application in ana- lyzing of the abnormal white blood cells and alarms capability of detection abnormality. Results Comparing with mi- croscopic examination,analyzer warning on low PLT had good accordance, Kappa value was 0. 97, U = 16.90, P~ 0.01. Warning on Left Shift had common accordance, Kappa value was 0.68 ,U~ 9.43, P〈0.01. Warnings on Imma- ture Gran, Atypical Lymph, Blasts, NRBC, PLT Clumps had poor accordance, Kappa value were 0. 35,0. 31,0. 27, 0. 28 and 0.25 respectively,U value were 7.15,4.89,4.66,4.17 and 4.55 respectively,P〈0.01. The criteria showed sensitivity was 95.70 % ,specificity was 81.16% and the accuracy was 85.67%. Conclusion The blood analyzer＇s a- larm message provides warning and screening roles,may reduce the labor intensity, raises the working efficiency, but cannot replace the microscopy completely. It is necessary to analyze generally for providing comprehensive, accurate and reliable survey report.