Development of multiplex real-time quantitative PCR for simultaneous detection of Chlamydia trachomatis,Mycoplasma hominis,Ureaplasma urealyticum,and Mycoplasma genitalium in infertile women |
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| Authors: | Sara Sadeqi Farhad Nikkhahi Amir Javadi Sonia Eskandarion Seyed Mahmoud Amin Marashi |
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| Affiliation: | 1. Department of Medical Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran;2. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran;3. Department of Community Medicine, Qazvin University of Medical Sciences, Qazvin, Iran;4. Vali-e-asr maternal, fetal, and Neonatal Research Center, Tehran University of Medical Sciences, Tehran, Iran;1. Bharati Vidyapeeth Deemed University Medical College, Pune- Satara Road, Dhanakawadi, Pune-411043, Maharashtra, India;2. Deenanath Mangeshkar Hospital, Pune, India;3. Jupiter Hospital, Pune, India;1. Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 110029, India;2. Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, 110029, India;3. Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, 110029, India;1. Institute of Infectious Diseases, Apollo Hospitals, Chennai, India;2. Department of Microbiology, Apollo Hospitals, Chennai, India;1. University of Health Sciences, Bursa Yüksek ?htisas Training and Research Hospital, Department of Gastroenterology, Bursa, Turkey;2. University of Health Sciences, ?i?li Hamidiye Etfal Training and Research Hospital, Medical Microbiology Clinic, Istanbul, Turkey;3. Erciyes University, Faculty of Veterinary Medicine, Department of Microbiology, Kayseri, Turkey;4. Nam?k Kemal University, Faculty of Medicine, Department of Gastroenterology, Tekirda?, Turkey;1. Dept of Microbiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, Tamil Nadu, India;2. UMR VITROME, Aix-Marseille University, Institut Hospitalo-universitaire Méditerranée Infection, Marseille, France;3. Department of Cardiology, Madras Medical College and Rajiv Gandhi General Hospital, Chennai, Tamil Nadu, India;4. Dept of Microbiology, Saveetha Dental College & Hospital, Chennai, Tamil Nadu, India;1. Dr. Lal Path Labs, Hyderabad, 500032, India;2. Department of Microbiology, Government Tirunelveli Medical College, Tirunelveli, 627011, Tamil Nadu, India |
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| Abstract: | PurposeSexually Transmitted Diseases (STDs) can cause sterility and many other problems for women planning pregnancy. Currently, almost 340 million people worldwide suffer from Sexually Transmitted Infections (STIs). This study made attempts to quickly identify STDs' most critical infectious agents using dedicated primers and probes.MethodsThe present study was done on the cervical samples of 200 infertile women. After extracting the total DNA of Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum, and Mycoplasma genitalium, quantitative methods were employed to determine the rate of target bacteria using multiplex real-time PCR.ResultsThe multiplex qPCR showed the rates of 47%, 16%, 46%, and 16.5% for Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum, and Mycoplasma genitalium in infertile women, respectively. In some patients, there were co-infections with two or three bacteria. The diagnostic approach used in our research could be employed as an alternative detection tool to identify the four most common STD-associated bacterial agents while detecting mixed infections.ConclusionsInfertile women with no biological problems could have their genital tract checked using this newly designed identification technique and get proper treatment for their infections as quickly as possible. |
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| Keywords: | Infertility Multiplex real-time polymerase chain reaction Sexually transmitted disease |
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