雪旺氏细胞移植至大鼠中脑损伤区域的研究

目的　探讨雪旺氏细胞移植对电针损伤的大鼠中脑网状结构的修复作用。方法　新生大鼠坐骨神经雪旺氏细胞体外培养 ,5溴 脱氧尿嘧啶 (BrdU)标记后移植至电针损伤的大鼠中脑网状结构。免疫组织化学方法观察不同时间BrdU、生长相关蛋白 43 (GAP 43 )的表达及髓鞘染色观察新生的髓鞘。结果　雪旺氏细胞移植后 8个月仍可见BrdU阳性细胞 ,且细胞数增加 2 3 % ,并主要向损伤侧大脑皮层迁移 ;与对照组相比 ,移植后 1个月损伤的中脑神经元GAP 43的表达有显著意义 (P <0 .0 5 ) ;在损伤的脑干区域可见新生的髓鞘。结论　雪旺氏细胞对损伤的中脑网状结构有促修复作用。

Transplantation of Schwann cells into injured brain stem in rats

Objective To explore the ability of grafted Schwann cells to promote restore of injured brain stem reticular structure in rats.Methods Schwann cells originated from sciatic nerves of 1 to 2-day-old rats were expanded and labeled with BrdU in vitro,transplanted into rat brain stem reticular structure injured by electric needle stimulus.Immunohistochemistry and myelin-staining were used to detect the expression of BrdU,GAP-43 and new myelination respectively.Results BrdU positive cells could be identified for up to 8 months and the number increased by about 23%,which mainly migrated toward injured ipsilateral cortex.The GAP-43 expression reached its peak one month after transplantation and was significantly increased as compared with control group (P<0.05).New myelination could be seen in destructed brain stem area.Conclusion The transplantation of Schwann cells could promote the restore of injured brain stem reticular structure.