白血病细胞多巴胺受体基因甲基化研究

目的：探讨多巴胺受体（ＤＲＤ４）基因第一外显子中ＮｏｔＩ酶切位点甲基化与急性非洒巴细胞白血病（ＡＭＬ）的关系。方法：应用Ｓｏｕｔｈｅｒｎ印记杂交与ＤＮＡ序列分析技术对ＡＭＬ患者骨髓标本进行分析。结果：在２７例白血病患者骨髓标本中１６例ＤＲＤ４基因甲基化（５９％）,其中初治化疗敏感患者ＤＲＤ４基因甲基化发生率为５０％（９／１８）,复发及难治患者甲基化发生率为７８％（７／９）,而９例正常人骨髓标本ＤＲ

Study on aberrant DNA methylation of dopamine D4 receptor gene in acute myeloid leukemia

Objective:To investigate aberrant DNA methylation of Not I site on exon I of dopamine D4 receptor (DRD4) gene in acute myeloid leukemia (AML).Methods:Southern Blot and DNA sequencing technique were performed on DNA from AML bone marrow samples. Results:Sixteen samples from 27 AML patients (59%) showed DNA methylation of DRD4 gene. Nine samples from 18 diagnostic chemosensitive patients (50%) and 7 samples from 9 refractory and relapse AML patients (78%) showed DNA methylation. But 9 normal bone marrow samples did not show any DNA methylation on DRD4 gene. Conclusion:The incidence of DRD4 gene methylation in AML was significantly higher than that in normal bone marrow ( P <0 05). There is potential importance of DRD4 gene methylation in initiation and development of AML.