1,8-桉叶油素调节自噬改善高糖诱导的内皮细胞损伤

目的探讨1,8-桉叶油素(1,8-Cineole)通过调节自噬来改善高糖(HG)诱导人主动脉内皮细胞(HAECs)损伤的保护作用。方法采用HG作用HAECs 60 h,不同剂量的1,8-Cineole进行干预,运用噻唑蓝(MTT)法检测细胞活力,乳酸脱氢酶(LDH)检测细胞毒性,Western blot法检测Beclin1、LC3-Ⅱ/Ⅰ、p62、caspase-3和caspase-9的蛋白表达,及使用自噬抑制剂氯喹(CQ)作用HAECs后,检测Beclin1、LC3-Ⅱ/Ⅰ、p62、caspase-3和caspase-9的表达情况。结果1,8-Cineole提高细胞活力,减少LDH含量,激活自噬和抑制细胞凋亡。与空白组比较,CQ组的Beclin1、LC3-Ⅱ/Ⅰ、p62、caspase-3和caspase-9表达增加,同时观察到CQ+HG+CH组与CQ+HG组比较时,以上蛋白差异均无统计学意义。结论1,8-Cineole对高糖诱导HAECs损伤具有保护作用,其作用与改善自噬流相关。

1,8-Cineole improves endothelial cell injury induced by high glucose via regulating autophagy

Aim To investigate the protective effect of 1,8-Cineole on the injury of human aortic endothelial cells(HAECs)induced by high glucose(HG)via regulating autophagy.Methods Cells were incubated with different doses of 1,8-Cineole followed by exposing to HG for 60 h,and MTT assay was used to analyse cell viability,lactate dehydrogenase(LDH)was used to detect cytotoxicity,and Western blot was used to detect Beclin1,LC3-Ⅱ/Ⅰ,p62,caspase-3 and caspase-9 expressions.Autophagy inhibitor(chloroquine,CQ)was treated on HAECs,and the expressions of Beclin1,LC3-Ⅱ/Ⅰ,p62,caspase-3 and caspase-9 were measured by Western blot.Results 1,8-Cineole increased cell viability,reduced the content of LDH,activated autophagy and inhibited apoptosis.Compared with control group,the expression of Beclin1,LC3-Ⅱ/Ⅰ,p62,caspase-3 and caspase-9 in CQ group increased.Simultaneously,the expression of above-mentioned between CQ+HG group and CQ+HG+CH group.Conclusions 1,8-Cineole has protective effect on the injury of HAECs induced by high glucose,and its effect is related to improving autophagy flux.