荔枝核总黄酮联合紫杉醇对前列腺癌耐药细胞的增殖抑制作用

目的探讨荔枝核总黄酮(Total flavonoids of litchi seed,TFLS)是否增强前列腺癌紫杉醇耐药细胞(Prostate cancer paclitaxel resistance cells,PCa-Txr)对紫杉醇(Paclitaxel,PTX)的敏感性,以及TFLS与PTX联合应用是否具有协同抑制作用。方法采用CCK-8法检测TFLS和PTX对PCa-Txr细胞及亲本细胞的增殖抑制作用;采用低细胞毒性剂量TFLS提前处理或与PTX同时作用PCa-Txr细胞来观察细胞对PTX敏感性;采用Chou T C推荐的联合方案来观察TFLS与PTX联合应用对PCa-Txr细胞的增殖抑制作用,并依据联合指数(Combination index,CI)判断TFLS与PTX联合应用对PCa-Txr细胞是否具有协同抑制作用。结果TFLS抑制PCa-Txr细胞及亲本细胞的增殖,并呈现时间和剂量依赖性。TFLS提前处理或与PTX同时作用PCa-Txr细胞均未能增加耐药细胞对PTX的敏感性。TFLS联合PTX明显抑制PCa-Txr细胞活性,CI值小于1,两药联合应用存在协同作用。结论TFLS抑制PCa-Txr细胞的增殖,TFLS联合PTX协同地抑制PCa-Txr细胞,但TFLS未能增加PCa-Txr细胞对PTX的敏感性。

The anti-proliferation effect of total flavonoids of litchi seed combined with paclitaxel on prostate cancer paclitaxel resistant cells

Aim To investigate the sensitization effect of total flavonoids of litchi seed(TFLS)to paclitaxel(PTX)on prostate cancer paclitaxel resistance(PCa-Txr)cells and the synergistic inhibitory effect of TFLS combined with PTX.Methods CCK-8 method was carried out to detect the inhibitory effect of TFLS and PTX on PCa-Txr cells as well as corresponding parental cells.PCa-Txr cells were either pre-treated or simultaneously treated with low cytotoxic dose of TFLS to observe the sensitivity of PCa-Txr cells to PTX.The synergistic inhibitory effect of TFLS combined with PTX on the proliferation of PCa-Txr cells were observed according to the method recommended by Chou T C.Combination index(CI)was used to determine whether TFLS and PTX had synergistic inhibitory effect.Results TFLS significantly inhibited the proliferation of PCa-Txr cells and parental cells in a certain time-and dose-dependent manner.The low dose of TFLS pre-treated or treated with PTX simultaneously failed to increase the sensitivity of PTX on PCa-Txr cells.TFLS combined with PTX significantly inhibited the proliferation of PCa-Txr cells with CI value less than one.Conclusions TFLS inhibits the proliferation of PCa-Txr cells,while TFLS combined with PTX has synergistic inhibitory effect on PCa-Txr cells.However,TFLS fails to increase the sensitivity of PCa-Txr cells to PTX.