| 人白细胞介素-24治疗人宫颈癌荷瘤裸鼠的实验研究 |
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| 引用本文： | 张婧,李莉. 人白细胞介素-24治疗人宫颈癌荷瘤裸鼠的实验研究[J]. 中国药物与临床, 2010, 10(2): 147-149 |
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| 作者姓名： | 张婧 李莉 |
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| 作者单位： | 山西医科大学第一医院妇产科,太原,030001 |
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| 摘 要： | 目的探讨人白细胞介素(hIL)-24基因重组质粒对人宫颈癌Siha细胞裸鼠移植瘤生长的抑制作用及其作用机制。方法建立荷宫颈癌Siha细胞裸鼠移植瘤模型,15只裸鼠随机分为3组:白细胞介素(IL)-24重组质粒组,空质粒组,磷酸盐缓冲液(PBS)组,根据肿瘤体积的生长情况绘制肿瘤生长曲线;处死裸鼠后切取瘤体并称质量,计算肿瘤抑制率;用流式细胞仪检测细胞周期。结果体内实验表明,IL-24重组质粒能够抑制肿瘤的生长,IL-24重组质粒组移植瘤的体积明显小于空质粒组和PBS组(P<0.05),空质粒组和PBS组移植瘤体积则差异无统计学意义(P>0.05)。IL-24重组质粒组抑瘤效果显著,其抑瘤率为56.5%,差异有统计学意义(P<0.05),空质粒组抑瘤率为1.3%,差异无统计学意义(P>0.05)。经流式细胞术检测,与空质粒组和PBS组比较,IL-24重组质粒组G0/G1期细胞增多(P<0.05),S期细胞减少,G2/M期细胞减少(P<0.05),细胞被阻滞在G0/G1期,空质粒组和PBS组则差异无统计学意义(P>0.05)。结论于荷瘤裸鼠的瘤内注射IL-24重组质粒可抑制肿瘤生长,提示IL-24具有明显的体内抗肿瘤作用。
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| 关 键 词： | 白细胞介素24 质粒 宫颈肿瘤 肿瘤,实验性 |
Therapeutic effect of IL-24 for human cervical cancer BALB/C in Nude mice |
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| ZHANG Jing,LI Li. Therapeutic effect of IL-24 for human cervical cancer BALB/C in Nude mice[J]. Chinese Remedies & Clinics, 2010, 10(2): 147-149 |
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| Authors: | ZHANG Jing LI Li |
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| Affiliation: | ZHANG Jing,LI Li.Department of Gynecology,First Hospital of Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To evaluate the growth inhibition activity and underlying mechanisms of IL-24 on human cervical cancer Siha cells in BALB/C nude mice.Methods After the model of human cervical cancer xenografts was established in nude mice,15 animals were randomly divided into 3 groups:IL-24 recombinant plasmid group,empty-vector plasmid group,and PBS group.Tumor growth curve was generated based on changes in tumor volume.On the 15th day after treatment,the nude mice were sacrificed,and tumor tissues were collecte... |
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| Keywords: | Interleulein 24 Plasmids Cervix neoplasms Neoplasms experimental |
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