胆结石患者胆汁幽门螺杆菌免疫印迹检测分析

目的 研究幽门螺杆菌感染与胆石形成的关系。方法 采用酶链免疫法(ELASA)对胆结石患者血清及胆汁免疫球蛋白IgG、IgA、IgM标本进行检测，对血清学及胆汁IgG均阳性患者的胆汁，采用免疫印迹法(Western Blot)进行幽门螺杆菌感染相关蛋白检测。结果 50例患者血清学阳性35例(70．0％)，胆汁阳性29例(58．0％)。血清学及胆汁均阳性24例(480％)。血清学及胆汁均阳性24例中，Cag A检出17例(70．8％)，Vac A检出11例(45．8％)。Ure B及Ure A分别为18例(75．0％)和16例(66.7％)。同时检出幽门螺杆菌细胞空泡毒素(Vac A)相关亚单位37kD、35kD糖蛋白。结论 胆汁中存在多种幽门螺杆菌感染相关蛋白，并可能参与胆结石的形成。

WESTERN BLOTTING OF HELICOBACTER PYLORI IN BILE OF PATIENTS WITH CHOLELITHIASIS

Objective To investigate relationship of helicobacter pylori(H.pylori) infection with gallstone formation. Methods Serum and IgG,IgA,IgM in bile sample was detected by enzyme-linked immunosorbent assays(ELIAS),and Western blotting was performed to detect H.pylori associated protein in both serum and bile samples showing IgG positive. Results IgG showed positive in 35(70.0%) of 50 serum samples, 29(58.0%) of 50 bile samples showed positive. 24 showed positive both in serum and bile samples, of which 17(70.8%) contained anti-CagA antibodies, 11(45.8%) contained VacAs. UreB and UreA were detected in 18(75.0%) and 16(66.7%) samples respectively. There were also Vacuolating cytotoxin associated 37kD and 35 kD glycoportein found in bile samples of patients with cholelithiasis. Conclusion It is suggest that presence associated protein of H.pylori infection in most bile samples may be represent risk factors for the formation of gallstone.