双波长HPLC法同时测定夏枯草中8种成分的含量

目的: 建立一种同时测定夏枯草中咖啡酸、对香豆酸、东莨菪内酯、迷迭香酸、秦皮乙素、芦丁、金丝桃苷、槲皮素8种成分含量的高效液相色谱方法。方法: 采用InertSustainTM ODS C18色谱柱（250.0 mm×4.6 mm，5 μm），流动相为乙腈-0.1%乙酸水溶液，梯度洗脱，流速1 mL/min，柱温30 ℃，检测波长为325 nm和360 nm。对所建立的HPLC方法进行线性关系考察、精密度试验、稳定性试验、重复性试验、加样回收率试验等方法学考察。结果: 8种成分在1~100 μg/mL范围内线性关系良好，平均加样回收率为98.63%~102.36%，精密度、稳定性、重复性试验的RSD均小于3%。结论:本方法能同时测定夏枯草中8种成分的含量，简单高效、准确可靠、重复性好，可用于夏枯草的质量控制。

Simultaneous determination of eight components in Prunella vulgaris L. by dual wavelength HPLC#br#

Objective To establish a HPLC method for simultaneous determination of caffeic acid, p coumaric acid, scopoletin, rosmarinic acid, esculetin, rutin, hyperin and quercetin in Prunella vulgaris L.. Methods The separation was performed on a InertSustainTM ODS C18 column （250.0 mm×4.6 mm, 5 μm), with mobile phase consisted of acetonitrile-0.1% acetic acid solution flowing at 1.0 mL/min in a gradient elution manner. The column temperature was 30 ℃ and the detection wavelength was set at 325 nm and 360 nm. The established HPLC method was investigated by linear relationship test, precision test, stability test, repeatability test and recovery test. Results The eight components showed good linear relationship in the range of 1-100 μg/mL, and the average recoveries were from 98.63% to 102.36%. Relative standard deviations of precision test， stability test and repeatability test were all lower than 3%. Conclusion This simple and reliable method may be applied to accurately determine the contents of eight components, which could be used for the quality control of Prunella vulgaris L..