2型糖尿病患者清道夫受体CD36基因多态性及其在单核细胞的表达

目的 探讨清道夫受体CD36多态性与2型糖尿病亚临床动脉粥样硬化(AS)发生的关系,了解2型糖尿病患者外周血单核细胞表面CD36的表达情况, 并探讨影响CD36表达的相关因素.方法 PCR-RFLP方法检测470例湖南地区汉族2型糖尿病患者及 220 名无糖尿病对照组D36(CD36-rs1984112、CD36-T620C位点)基因多态性,比较它们基因型及等位基因频率的差异.以流式细胞仪检测102例2型糖尿病患者的外周血单核细胞表面CD36蛋白表达荧光强度,比较2型糖尿病无AS组、2型糖尿病亚临床AS组CD36的表达.多元线性回归分析CD36表达的影响因素.结果 2型糖尿病患者与健康受试者CD36(CD36-rs1984112位点、CD36-T620C位点)基因型及等位基因频率进行比较,其差异均无统计学意义;2型糖尿病亚临床AS组外周血单核细胞CD36的平均荧光强度(MFI)高于2型糖尿病无AS组(1 382±659 vs 1 173±340,P＜0.05).影响2型糖尿患者CD36表达的因素有年龄(P=0.005)、性别(P=0.021)、收缩压(P=0.027),标化偏回归系数分别为0.28、0.31、-0.21;影响男性CD36表达的因素为年龄(P=0.002);影响女性CD36表达的因素为舒张压(P=0.001).结论 在中国南方汉族人群中,CD36-rs1984112及T620C位点可能不是功能性多态位点.2型糖尿病亚临床AS组外周血单核细胞表面CD36蛋白表达较无AS组高.增龄、男性、低收缩压可使CD36蛋白表达增加.

Polymorphisms of scavenger receptor CD36 and its expression of monocyte surface in type 2 diabetic patients

Objective To investigate the role of polymorphisms of scavenger receptor class B gene CD36 in affecting the progress of subclinical atherosclerosis (AS) and the associated factors affecting the expression of CD36 on the surface of peripheral blood monouclear cells (PBMC) and the association between CD36 expression and progress of subclinical AS in type 2 diabetic patients.Methods CD36 polymorphisms (CD36-rs1984112, CD36-T620C) were typed by PCR-RFLP in 470 cases of type 2 diabetes mellitus and 220 non-diabetic controls of Hans in Hunan area.The genotypes and allele frequencies were compared between cases and controls.Fluorescence intensity of CD36 on the surface of PBMC was analyzed in 102 cases of type 2 diabetes mellitus by flow cytometry and was compared between the patients without AS and the patients with subclinical AS.Multiple linear regression was applied to evaluate the relevant factors contributing to CD36 expression.Results The genotypes and allele frequencies of CD36-rs1984112 in type 2 diabetes mellitus were not significantly different between cases and controls (P＞0.05), either did CD36-T620C (P＞0.05).The mean florescence intensity (MFI) of CD36 in type 2 diabetics with subclinical AS was higher than that without AS (1 382±659 vs 1 173±340, P＜0.05).Factors affecting the CD36 expression were: age (P=0.005), gender (P=0.021), systolic blood pressure (SBP) (P=0.027), standardized coefficients Beta was 0.28, 0.31 and -0.21, respectively.Age contributed to the CD36 expression level in males (P=0.002) and diastolic blood pressure in females (P=0.001) respectively.Conclusion CD36-rs1984112 and T620C seem not to be a functional polymorphism sites in Hans of Hunan, southern China.CD36 expression level is higher in type 2 diabetics with subclinical AS in contrast with those without AS.CD36 expression on PBMC surface is higher in aged males with lower SBP.