DSCAM在大鼠骨髓间质干细胞分化为神经细胞中的表达变化

目的：研究唐氏综合征细胞粘附分子（DSCAM）在大鼠骨髓间质干细胞（MSCs）分化为神经细胞中的作用。方法：在建立黄芩苷诱导大鼠MSCs分化为神经细胞的基础上，采用免疫细胞化学法、Western blot法等检测DSCAM的表达变化；同时采用RNA干扰技术，观察DSCAM-siRNA转染MSCs后诱导分化情况。结果：诱导前大鼠MSCs不表达DSCAM；预诱导液诱导24 h，MSCs开始少量表达DSCAM（1.71±0.67）%；黄芩甙诱导 6 h，部分表达DSCAM（15.79±4.24）%；诱导后3 d，DSCAM表达最高（53.16±5.94）%；诱导后 6 d，DSCAM表达明显下降（28.99±6.72）%。DSCAM-siRNA转染MSCs，DSCAM表达显著下降。而且，诱导前MSCs不表达神经细胞特异性标记蛋白β-III-tubulin；诱导6 h，β-III-tubulin表达为（1.40±0.79）%，诱导3 d达到（41.59±3.17）%，诱导 6 d 为（59.11±4.76）%。但是， DSCAM-siRNA转染MSCs，诱导3 d，6 d，β-III-tubulin蛋白的表达显著下降，分别为（28.57±2.91）%和（43.90±12.31）%。结论：DSCAM可能在MSCs分化为神经细胞中起到重要的作用。［中国当代儿科杂志，2009，11（6）：486-489］

Effects of DSCAM on differentiation of rat marrow mesenchymal stem cells into neurons in vitro

Objective To study the effects of Down syndrome cellular adhesion molecule (DSCAM) on differentiation of rat marrow mesenchymal stem cells (MSCs) into neurons in vitro.Methods MSCs from Sprague-Dawley rats were induced into neurons by baicalin. The expression of DSCAM before and after induction was evaluated by immunocytochemical staining and Western blot assay. After knockdown of DSCAM by siRNA transfection, the differentiation rate of neurons derived from MSCs was measured.Results Before induction, the ex...