氧化低密度脂蛋白在慢性梗阻性黄疸大鼠肝脏中的表达与意义

目的 探讨氧化低密度脂蛋白在慢性梗阻性黄疸大鼠中的表达与肝脏纤维化的关系.方法 60只SD大鼠被随机分成对照组（A组）和慢性梗阻性黄疸组（B组）两组,每组每时段6只.分别于造模后第1、2、3、4、5周抽取大鼠静脉血检查肝功能,显微镜目镜刻度下测量大鼠胆总管直径,免疫荧光法检测氧化低密度脂蛋白在肝脏的表达,放射免疫法检测肝纤维化三项,光学显微镜观察肝组织的病理改变.第4周行胆总管十二指肠吻合再通.结果 慢性梗阻性黄疸组大鼠术后总胆红素升高,胆总管直径扩张,组织病理学检查显示肝细胞变性,并有纤维增生性改变.与对照组比较,慢性梗阻性黄疸组肝组织氧化低密度脂蛋白的表达增强,肝纤维化三项术后第3周起明显升高.胆总管与十二指肠端侧吻合再通后黄疸缓慢消退.术后第3周氧化低密度脂蛋白的表达及肝纤维化三项差异有统计学意义（P 〈 0.05）.结论 慢性梗阻性黄疸大鼠肝组织氧化低密度脂蛋白的表达增强,与肝脏的纤维化的程度相关.

Expression and significance of Ox-LDL in chronic obstructive jaundice rat model

Objective To study the relation between expression of Ox-LDL and liver fibrosis in chronic obstructive jaundice rats model. Methods Sixty Sprague-Dawley rats were randomly and equally divided into control and chronic obstructive jaundice group. Each group was further divided into five subgroups for testing at different time points. Liver function was determined and the diameter of the common bile duct was measured under a microscope. Expression of Ox-LDL was tested by immunofluore-scence and three item liver fibrosis was also measured by radioimmunodetection.Pathological changes in liver tissue were also observed by optical microscopy at weeks 1, 2, 3, 4, and 5 after model induction. Choledochoduodenostomy was performed at 4th week. Results Jaundice was mild in rats with chronic obstructive jaundice, Meanwhile the diameter of the common bile duct increased, and obvious degeneration and hyperplasia of liver cells were noted. There were also undulatory in expression of Ox-LDL and three item liver fibrosis. Jaundice slowly subsided after choledochoduodenostomy. Total bilirubin, the diameter of the common bile duct, expression of Ox-LDL and three item liver fibrosis differed significantly at different time points between rats with control group and chronic obstructive jaundice group (both P < 0.05). Conclusions There are significantly relation between expression of Ox-LDL and hepatocellular injury, particularly with fibrosis in chronic obstructive jaundice rats model. Further studies are needed to evaluate the potential effects of ox-LDL.