HPLC对肺结核患者利福平血药浓度的监测

目的 建立肺结核患者给予利福平后血药浓度的监测方法,为临床合理用药提供参考.方法 高效液相法,色谱柱为安捷伦C18柱,(5μm,4.6×250 mm);流动相为甲醇:乙腈:0.04 mol×L-1 KH2PO3( 30:30:40);流速1.2 ml/min,检测波长为340 nm;柱温20℃.结果 血浆中利福平在0.30 ～24 μg/ml范围内浓度与峰面积线性关系良好,r=0.9995,平均回收率为98.87％.140例肺结核患者静滴或口服利福平450 mg后,其中126例血药浓度检测结果在0.50～12.75 μg/ml之间,12例血药浓度检测结果0.30～0.5 μg/ml之间,2例血药浓度小于0.3μg/ml.结论 该方法准确、灵敏,专属性强,重现性好,适用于治疗药物监测,可为临床合理用药提供数据参考.

Monitoring of plasma rifampicin concentrations in patients with pulmonary tuberculosis by HPLC

Objective To monitor plasma rifampicin concentrations in patients with pulmonary tuberculosis by HPLC.Methods The assay was conducted on C18 GL sciences column（5μm,4.6×250 mm）with the mobile phase of the mixture of CH₃OH-CH₃CN-0.04 mol/L KH₂PO₃（30:30:40）at a rate of 1.2 ml/min,the UV detection wavelength was 340 nm.The column temperature was 20℃. Results A good linearity was obtained from 0.30～24μg/ml with a correlation coefficient of 0.999 5.The average recovery of the assay was 98.87%.Among 140 patients administrated with 450 mg rifampicin orally or intravenously,the plasma drug concentration ranged 0.50～12.75μg/ml in 126 cases,ranged 0.30～0.5μg/ml in 12 cases and was less than 0.3μg/ml in 2 cases.Conclusion The method applied in this study is accurate and sensitive,which can be used to monitor plasma rifampicin concentration for rational drug administration in clinical practice.