Zebularine对K562细胞的增殖及凋亡的影响

[目的]探讨药物zebularine对白血病细胞株K562增殖及凋亡的影响.[方法]选择白血病细胞株K562,试验分为对照组(未加入zebularine组)、实验组 (zebularine终浓度分别为 250 μM,500 μM)组3个组.MTT试剂盒检测12 h、24 h、36 h、48 h和 72 h后K562细胞...

Effect of Zebularine on the Proliferation and Apoptosis of K562 Cells

[Obiective] To understand the effect of zebularine on the proliferation and apoptosis of leukemia cells K562 and explore its tumor suppressor mechanism and value in the treatment of leukemia. [Methods] The K562 leukemia cells were selected and divided into the control group（without zebulaine） and the experimental groups（zebularine with the final concentration of 250μM and 500μM, respectively）. MTT assay kit was used to detect the OD570 of K562 cells after 12h, 36h, 24h, 48h and 72h. Annexin V/FITC kit and cell cycle kit were used to detect the apoptosis of K562 cells at 48h after drug intervention. [Results] At 48h after drug intervention, the activity of k562 markedly reduced with the increasing of concentration of zebularine（ P 〈0.01）. Compared with the control group, the proportion of early apoptotic cells and apoptotic peak（APO） value in the experimental groups after 48h of drug intervention increased obviously, but there was significant difference（ P 〈0.01）. Compared with 250μM group, the proportion of early apoptotic cells and APO peak value of 500μM group increased obviously, and there was significant difference between two groups （ P 〈0.01）. [Conclusion]Zebularine can inhibit the proliferation of K562 cells and promote the apoptosis in a dosedependent manner.