| 曲古抑素A激活FAS途径促使A549/CDDP细胞凋亡 |
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| 引用本文： | 伍俊,易震南,胡成平. 曲古抑素A激活FAS途径促使A549/CDDP细胞凋亡[J]. 重庆医学, 2011, 40(23): 2294-2296,后插一. DOI: 10.3969/j.issn.1671-8348.2011.23.003 |
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| 作者姓名： | 伍俊 易震南 胡成平 |
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| 作者单位： | 伍俊,易震南,Wu Jun,Yi Zhennan(广东医学院呼吸疾病研究所,广东湛江,524023);胡成平,Hu Chengping(中南大学湘雅医院呼吸内科,长沙,410008) |
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| 摘 要： | 目的 探讨曲古抑素A(TSA)是否可以诱导FAS的表达,促进人肺腺癌耐顺铂细胞株A549/CDDP细胞凋亡,揭示TSA诱导A549/CDDP细胞凋亡的可能机制.方法 Hoechst 33258荧光染色观察细胞凋亡,流式细胞仪检测细胞凋亡和细胞周期变化,间接免疫荧光检测FAS表达,蛋白印迹法检测FAS、半胱氨酸天冬氨酸蛋...
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| 关 键 词： | 曲古抑素A 细胞凋亡 FAS 半胱氨酸天冬氨酸蛋白酶8 |
TSA prompts A549/CDDP cell apoptosis by activating FAS-pathway |
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| Wu Jun,Yi Zhennan,Hu Chengping. TSA prompts A549/CDDP cell apoptosis by activating FAS-pathway[J]. Chongqing Medical Journal, 2011, 40(23): 2294-2296,后插一. DOI: 10.3969/j.issn.1671-8348.2011.23.003 |
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| Authors: | Wu Jun Yi Zhennan Hu Chengping |
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| Affiliation: | Wu Jun1,Yi Zhennan1,Hu Chengping2(1.Institute of Respiratory Diseases,Guangdong Medical College,Zhanjiang 524023,China,2.Department of Respiratory Diseases,Xiangya Hospital of Central South University,Changsha 410008,China) |
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| Abstract: | Objective To investigate the effect of trichostatin A(TSA)on FAS-dependent pathway for studying the mechanism of TSA-induced A549/CDDP cell apoptosis.Methods Cell apoptosis was analyzed by Hoechst 33258 fluorescence dye and flow cytometry.The changes of Caspases-8 and FAS in A549/CDDP cells were detected with Western blotting and indirect immunofluorescence assay.Results After treatment with TSA,A549/CDDP cells showed typical apoptotic morphological character.Using flow cytometry,the apoptotic percentage in... |
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| Keywords: | FAS |
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