胚胎干细胞基因Nanog，Oct-4基因在前列腺癌中的表达及意义

【目的】探讨Nanog基因和Oct-4基因在良性前列腺增生（BPH），前列腺上皮内瘤变（PIN）及前列腺癌（PCa）中的表达及其在PCa发生发展中的作用。【方法】用免疫组织化学法检测BPH（n=30）、PIN（n=8）厦、PCa（n=94）中胚胎干细胞相关基因Nanog，Oct-4的表达水平，并比较三者之间的差异。【结果】Nanog基因和Oct-4基因在BPH和PIN组织中无明显差异（P〉0．05）。PCa组织中的Nanog基因和Oct-4基因明显高于BPH及PIN（P〈0．05）。随着PCa组织Gleason评分的增加，肿瘤中干细胞相关基因Nanog，Oct-4的表达也明显增多（P〈0．05）。【结论】Nanog，Oct-4基因在前列腺癌中均有表达，其表达水平与肿瘤的组织学分级相关。

Expression of Embryonic Stem Cell Genes Nanog and Oct-4 in Human Prostate Cancer and Its Clinical Significance

Objective]To explore the expression of Nanog and Oct-4 genes in benign prostatic hyperplasia（BPH）, prostatic intraepithelial neoplasm（PIN） and prostatic eancer（PCa） and the role in the genesis and development of PCa. Methods] The expression of embryonic stem cell genes Nanog and Oct-4 were detected by immunohistochemistry method in 30 BPH patients, 8 PIN patients and 94 PCa patients. The difference among three grou.ps was compared. Results] There was no obvious difference in the expression of Nanog and Oct-4 between BPH and PIN tissues（ P 〉0.05）. The expression of Nanog and Oct-4 in Pca tissues were obviously higher than those in BPH and PIN tissues（ P 〉0.05）. With the increasing of Gleason Score, the expression of Nanog and Oct-4 increased（ P 〈0.05）. Conclusion] Nanog and Oct-4 genes are expressed on prostatic cancer, and their expression levels are related to the histological grade of the tumor.