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内障丸对青春期PCOS大鼠卵巢颗粒细胞凋亡因子Bcl-2及Bax的影响
引用本文:姚美玉,李祥,陈皇珍. 内障丸对青春期PCOS大鼠卵巢颗粒细胞凋亡因子Bcl-2及Bax的影响[J]. 实用中医药杂志, 2009, 25(11): 716-717
作者姓名:姚美玉  李祥  陈皇珍
作者单位:1. 黑龙江中医药大学附属第一医院妇科,黑龙江,哈儿滨,150040
2. 黑龙江省五常市中医院,黑龙江,五常,150200
3. 黑龙江中医药大学,黑龙江,哈尔滨,150040
摘    要:目的:探讨内障丸对青春期PCOS大鼠卵巢颗粒细胞凋亡因子Bcl-2及Bax表达的影响。方法:以23日龄幼年SD清洁雌性大鼠为研究对象,应用皮下埋植左炔诺孕酮硅胶棒联合皮下注射HCG法建立青春期PCOS动物模型,运用免疫组织化学SABC方法及光、电镜技术等方法,检测大鼠造模前后及给药前后卵巢颗粒细胞Bcl-2,Bax的表达情况。结果:造模后模型组颗粒细胞Bcl一2,Bax,Bcl-2/Bax表达水平与正常对照组差异明显,运用阳性目标面密度进行比较,经治疗后内障丸高、中、低剂量组Bcl-2表达均强于Bax。其中高剂量组的表达与正常组接近。在对照组中,Bax表达较Bcl-2强。结论:内障丸可以抑制多囊卵巢大鼠卵巢颗粒细胞凋亡及卵泡闭锁,而且可以使多囊卵巢大鼠卵泡闭锁时间提前,减轻卵巢多囊样改变,从而改善卵巢功能。

关 键 词:内障丸  大鼠  青春期PCOS  细胞凋亡  Bcl-2  Bax  影响

Effect of Rou Zhang Pills on Apoptosis Factor Bcl-2 and Bax of Ovarian Granulosa Ceils in Adolescent PCOS Rats
Yao Meiyu,Li Xiang,Chen Huangzhen. Effect of Rou Zhang Pills on Apoptosis Factor Bcl-2 and Bax of Ovarian Granulosa Ceils in Adolescent PCOS Rats[J]. Journal of Practical Traditional Chinese Medicine, 2009, 25(11): 716-717
Authors:Yao Meiyu  Li Xiang  Chen Huangzhen
Abstract:Objective:To investigate the influence onovary granular cell apoptosis factor Bcl -2 and Bax ofpuberty PCO rats treated with Neizhang Pill, Methods : Take the 23 - day - old immature female SD rats as the object of study, progesterone - synchronized immature rats were used as the animal model for PCO by in -jecting HCG. Compared with normal control group,investigate the changement of the modelgroup's ovary granular cell apoptosis factor Bcl -2 and Bax. Results:The treatment group's expressions of Bcl -2 and Bax not differ from the control group's. Conclusions:Neizhang Pill could promote ovulation by inhibit ovary granular cell apoptosis and follicle atresia.
Keywords:Bcl-2  Bax
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