胃癌患者血清中骨桥蛋白和CD44v6检测的临床意义

目的检测胃癌患者血清中骨桥蛋白(osteopontin,OPN)和CD44v6(CD44 splice variant 6)的表达水平,探讨二者在胃癌诊断和病情监测方面的价值。方法应用酶联免疫吸附试验检测60例胃癌患者(胃癌组),42例胃溃疡患者(胃溃疡组),33例健康体检正常者(健康体检组)血清中OPN和CD44v6的水平。结果胃癌组血清中OPN和CD44v6的水平均高于溃疡组及健康体检组,差异均有统计学意义(P<0.01);胃癌患者血清OPN和CD44v6的水平均与胃癌的临床分期、肿瘤大小、淋巴结转移及远处转移相关(P<0.05)。胃癌患者血清中OPN与CD44v6的水平呈正相关(r=0.8645,P<0.01);二者联合检测敏感性提高至92.5%。结论血清OPN和CD44v6的水平可作为胃癌诊断和病情监测的临床指标,二者联合检测提高了胃癌诊断敏感性,可作为筛查指标用于胃癌的筛检。

Detection of Osteopontin and CD44v6 in Patients with Gastric Cancer and Its Clinical Significance

Objective To detect the expression levels of osteopontin(OPN) and CD44v6 in serum of gastric cancer patients and to investigate their clinical value in diagnosing and monitoring gastric cancer.Methods The levels of OPN and CD44v6 in 60 serum samples of gastric cancer patients,42 serum samples of gastric ulcer patients and 33 serum samples of healthy persons were measured by enzyme-linked immuno-sorbent assay.Results The expression levels of OPN and CD44v6 in serum of gastric cancer patients were significantly higher than those of gastric ulcer patients and healthy examination persons(P<0.01);The expression levels of OPN and CD44v6 in serum of gastric cancer patients were associated with PTNM stage,the size of the tumor,the metastasis of lymph node and distant metastasis(P<0.05);The expression levels of OPN and CD44v6 in serum of gastric cancer patients were positively correlated with each other(r=0.8645,P<0.01);The sensitivity of combined detection of the two markers was improved to 92.5%.Conclusion The assay of OPN and CD44v6 in serum of gastric cancer patients is helpful in diagnosing and predicting the risk for metastasis and prognosis in gastric cancer.The combined detection of the two markers can improve the detection rate and can be taken as a screening method for gastric cancer.