丁酸钠对胃癌细胞系生长的抑制作用及其机制

目的:观察丁酸钠(NaB)对体外培养的胃癌细胞系BGC823的生长抑制作用,并进一步探讨其作用机制。方法:应用MTT法检测细胞增殖活性;流式细胞仪检测细胞周期分布;Annexin V-FITC染色法检测细胞凋亡率。结果:不同浓度NaB处理胃癌细胞BGC82324~96h后,细胞增殖显著受到抑制,并呈浓度(P<0.01)及时间(P<0.05)依赖性;1.0,3.0,5.0mmol/L NaB处理72h后,BGC823细胞G0/G1期细胞数量显著增加,S期细胞数量显著降低(P<0.01);各浓度组细胞凋亡率均显著增加(P<0.01)。结论:NaB对胃癌细胞系BGC823具有生长抑制作用,其机制与阻滞细胞周期及诱导凋亡有关。

Effect of sodium butyrate on proliferation of human gastric cancer cell line and its mechanism

Objective:To investigate the effect of sodium butyrate(NaB),one of histone deacetylase inhibitors(HDACI),on the proliferation of human gastric cancer cell line BGC823 and its mechanism.Methods:BGC823 cells cultured in RPMI 1640 were treated with 0.5,1.0,3.0,and 5.0 mmol/L of NaB.MTT assay,flow cytometry,and Annexin V-FITC staining were performed to determine the cell proliferation activity,cell cycle,and apoptotic rate.Results:After BGC823 cells were treated with different concentrations of NaB for 24 to 96 hours,the cell proliferation activity was inhibited in a dose-and time-dependent manner(P<0.01,P<0.05).The number of cells in G0/G1 phase significantly increased and the number of cells in S phase significantly decreased after the cells were treated with 1.0,3.0,and 5.0 mmol/L of NaB for 72 hours(both P<0.01).The apoptotic rate significantly increased in cells treated with different concentrations of NaB(all P<0.01).Conclusion:NaB could inhibit the growth of human gastric cancer cell line BGC823 by blocking cell cycle and inducing apoptosis.