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| 用Western Blot和原位杂交法研究Shh在小鼠牙胚钟状晚期的表达 | |
| 引用本文： | 周彦秋,林久祥,贾弘褆. 用Western Blot和原位杂交法研究Shh在小鼠牙胚钟状晚期的表达[J]. 中华口腔医学杂志, 2003, 38(3): 210-212,I001 |
| 作者姓名： | 周彦秋 林久祥 贾弘褆 |
| 作者单位： | 1. 100081,北京大学口腔医学院正畸科 2. 100081,北京大学基础医学院生物化学与分子生物学系 |
| 基金项目： | 国家自然科学基金资助项目(3 0 1710 2 3 ) |
| 摘 要： | 目的 从形态学和半定量分析两方面研究Shh(Sonic Hedghog)基因在小鼠牙胚钟状晚期的表达，探讨Shh在牙胚钟状晚期的生物学作用。方法 取出生后1、2、3、5和7d(P1,P2,P3,P5,P7)的Bal b/c纯系小鼠牙胚，用Western印迹方法检测Shh的表达量；原位杂交检测Shh在出生后1d和3d天乳鼠牙胚中的表达部位与强度。结果 钟状晚期Shh特异表达于成釉细胞层，其强度随出生天数增加而减弱；随着成釉细胞发育成熟，44500Shh表达量逐渐降低，P1的吸光光度值为P7的5.5倍，P1，P2与P3可检测出活性Shh-N片段。结论 Shh在钟状晚期特异地表达于成釉细胞层，其表达量与成釉细胞的功能状态有关。 |
| 关 键 词： | Western Blot 原位杂交法 Shh 小鼠 牙胚发育 钟状晚期 |
| 修稿时间： | 2002-01-10 |
The expression of Sonic Hedgehog during late bell stage by Western Blot and in situ hybridization |
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| ZHOU Yan qiu ,LIN Jiu xiang,JIA Hong ti. The expression of Sonic Hedgehog during late bell stage by Western Blot and in situ hybridization[J]. Chinese journal of stomatology, 2003, 38(3): 210-212,I001 | |
| Authors: | ZHOU Yan qiu LIN Jiu xiang JIA Hong ti |
| Affiliation: | Department of Orthodontics, Peking University School of Stomatology, Beijing 100081, China. |
| Abstract: | Objective To investigate the expression and biological effect of Shh during late bell stage by morphological and semi quantitative analysis Methods Tooth germs were selected from new born Bal b/c mouse (P1, P2, P3, P5, P7) Semi quality of Shh was measured by Western Blot and the expression place and strength of Shh were observed by in situ hybridization Results Shh was expressed in the ameloblast layer during late bell stage; the expression strength was high in secretive period and decreased with development; the active N section was detectable before P3 Conclusion Shh expresses specially in the ameloblast layer in late bell stage, and expression quality is related tothe function of ameloblasts |
| Keywords: | Gene expression Tooth germ Development Sonic Hedgehog |
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