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| HO-1在姜黄素拮抗乙醇所致肝细胞氧化损伤过程中的作用 | |
| 引用本文： | 欧阳英英,李珂,荣爽,姚平,杨年红,孙秀发,刘烈刚. HO-1在姜黄素拮抗乙醇所致肝细胞氧化损伤过程中的作用[J]. 华中科技大学学报(医学版), 2010, 39(1). DOI: 10.3870/j.issn.1672-0741.2010.01.020 |
| 作者姓名： | 欧阳英英 李珂 荣爽 姚平 杨年红 孙秀发 刘烈刚 |
| 作者单位： | 华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉,430030 |
| 基金项目： | 教育部新世纪优秀人才支持计划项目 |
| 摘 要： | 目的 研究血红素氧化酶-1(HO-1)在姜黄素拮抗乙醇氧化损伤过程中所起的作用.方法 通过亚细胞分离法提取微粒体,测定姜黄素预作用大鼠原代肝细胞中HO-1的活性,确定姜黄素对HO-1的诱导情况.通过抑制剂ZnPPⅨ和诱导剂Hemin的加入,抑制和诱导HO-1的表达与活性,通过检测细胞培养上清液中谷草转氨酶(AST)、乳酸脱氢酶(LDH)活性以及肝细胞的氧化-抗氧化指标,确定HO-1在姜黄素拮抗乙醇所致肝细胞氧化损伤过程中的作用.结果姜黄素能够诱导HO-1的活性,在姜黄素15 μmol/L和提前1 h作用时HO-1水平达到高峰.且HO-1的活性和表达与肝细胞的抗氧化水平明显相关.结论 姜黄素对肝细胞有明显的保护作用,主要表现在提高细胞HO-1的活性及表达水平. |
| 关 键 词： | 姜黄素 血红素氧化酶-1 抗氧化酶 氧化损伤 |
Involvement of Heme Oxygenase-1 in the Protective Actions of Curcumin against Ethanol-induced Oxidative Damage in Rat Primary Hepatocytes |
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| Ouyang Yingying,Li Ke,Rong Shuang,Yao Ping,Yang Nianhong,Sun Xiufa,Liu Liegang. Involvement of Heme Oxygenase-1 in the Protective Actions of Curcumin against Ethanol-induced Oxidative Damage in Rat Primary Hepatocytes[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2010, 39(1). DOI: 10.3870/j.issn.1672-0741.2010.01.020 | |
| Authors: | Ouyang Yingying Li Ke Rong Shuang Yao Ping Yang Nianhong Sun Xiufa Liu Liegang |
| Affiliation: | Ouyang Yingying,Li Ke,Rong Shuang et al Department of Nutritional , Food Hygiene,School of Public Health,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,China |
| Abstract: | Objective To investigate the role of heme oxygenase-1(HO-1)in the protective actions of curcumin against ethanol-induced oxidative damage.Methods Microsomal HO-1 enzyme activities were determined in rat primary hepatocytes pretreated by curcumin.AST,LDH release and hepatic oxidative/antioxidant status were measured with or without ZnPPⅨ/Hemin as a classic HO-1 inhibitor/inducer,respectively.Results Curcumin could induce the HO-1 expression and enzyme activity,which was correlated with antioxidant levels in hepatocytes.HO-1 induction reached a peak under administration of 15 μmol/L curcumin for 1 h.Conclusion HO-1 induction by curcumin is contributed to the heptoprotective effects against ethanol-induced oxidative damage. |
| Keywords: | curcumin heme oxygenase-1 ethanol oxidative stress |
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