大孔吸附树脂纯化鬼针草总黄酮的工艺优选

目的优选大孔树脂纯化鬼针草总黄酮的工艺条件。方法以鬼针草总黄酮的吸附率和洗脱率为指标，通过静态吸附试验比较不同种大孔树脂对鬼针草总黄酮的吸附能力，筛选出合适的大孔树脂型号；通过单因素试验优选鬼针草总黄酮的纯化工艺参数。结果HPD400型大孔树脂纯化效果最好，其最佳工艺参数为药液中质量浓度0．5mg／mL，pH=4．0，吸附速率2BV／h，用9BV60％乙醇洗脱，洗脱速率3BV／h，经大孔树脂纯化后鬼针草提取液中总黄酮纯度由原来的24．47％提高至58．41％。结论HPD400型大孔树脂适用于鬼针草总黄酮的纯化，优选的纯化工艺稳定可行。

Optimization of Purification Technology for Total Flavonoids in Bidens bipinnata by Macroporous Adsorption Resin

Objective To optimize purification technology of total flavonoids in Bidens bipinnata by macroporous resin. Method With adsorption rate and desorption rate of total flavonoids as indexes, adsorption property of six different kinds of macroporous resins for total flavonoids in B. bipinnata was compared by static adsorption and desorpfion experiments, in order to select optimum macroporous resin. Single factor test was used to investigate adsorption and elution conditions of macroporous resin. Result HPD400 macroporous resin presented the best effect of purification. Its optimum purification parameters were as followings： the sample concentration 0.5 g/L,pH 4,sample flow rate 2 BV/h,eluted with 9 BV 60% ethanol at 3 BV/h. After purification by macroporous resin, purity of total flavonoids in ethanol extract ofB. bipinnata would be increased from 24.47% to 62.33%. Conclusion HPD400 macroporous resin was among the most suitable one for purification of total flavonoids in B. bipinnata. Optimized purification technology was stable and feasible.