| 涎腺多形性腺瘤p53基因突变的SSCP及核酸序列分析研究 |
| |
| 引用本文： | 王洁,董福生,雍平.涎腺多形性腺瘤p53基因突变的SSCP及核酸序列分析研究[J].中华口腔医学杂志,1999,34(5):298-300. |
| |
| 作者姓名： | 王洁 董福生 雍平 |
| |
| 作者单位： | 河北医科大学口腔医学院 |
| |
| 基金项目： | 卫生部优秀青年科技人才专项科研基金!96Q -0 61 |
| |
| 摘 要： | 目的 检测涎腺多形性腺瘤中ｐ５３基因第６外显子突变的密码及突变类型。方法 采用聚合酶链式反应－单股构型多态分析（ｐｏｌｙｍｅｒａｓｅ ｃｈａｉｎｊ ｒｅａｃｔｉｏｎ－ｓｉｎｇｌｅ－ｓｔｒａｎｄ ｃｏｎｆｏｒｍａｔｉｏｎａｌ ｐｏｌｙｍｏｒｐｈｉｓｍ，ＰＣＲ－ＳＳＣＰ）技术及ＤＮＡ核酸序列测定方法对涎腺多形性腺瘤进行研究。结果 在１１例ＰＣＲ－ＳＳＣＰ阳性的肿瘤中，第６外显子异常者６例。对其中４例肿
|
| 关 键 词： | 涎腺肿瘤 涎腺多形性腺瘤 p53基因 SSCP DNA |
p53 gene mutation determined by single strand conformational polymorphism and DNA sequencing analysis in pleomorphic adenoma of salivary gland |
| |
| WANG Jie,DONG Fusheng,YONG Ping,et al School of Stomatology,Hebei Medical University,Shijiazhuang.p53 gene mutation determined by single strand conformational polymorphism and DNA sequencing analysis in pleomorphic adenoma of salivary gland[J].Chinese Journal of Stomatology,1999,34(5):298-300. |
| |
| Authors: | WANG Jie DONG Fusheng YONG Ping School of Stomatology Hebei Medical University Shijiazhuang |
| |
| Institution: | School of Stomatology, Hebei Medical University, Shijiazhuang 050017. |
| |
| Abstract: | OBJECTIVE: To detect p53 exon 6 mutation pattern in pleomorphic adenoma of salivary gland. METHODS: Single-strand conformational polymorphism (SSCP) and DNA sequecing were used. RESULTS: SSCP analysis showed that 6 of 11 cases with SSCP positive were exon 6 abnormal. Comparison of the SSCP results with DNA sequecing, 4 cases with p53 exon 6 mutations were SSCP positive. Mutation pattern included base substitution (point mutation, G-->T, T-->G) and frame-shift mutation (base insertion and base loss). CONCLUSION: Poly-codon and poly-pattern mutations appeared in p53 exon 6. PCR-SSCP was a convenient method to detecte gene mutation. |
| |
| Keywords: | Adenoma pleomorphic Genes p53 Mutation Sequence analysis DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
