顺铂增强食管癌相关基因2蛋白对人食管癌EC9706细胞的增殖抑制和凋亡诱导作用

 摘要：目的:探讨食管癌相关基因2（esophageal cancer-related gene 2，ECRG2）蛋白联合顺铂（cisplatin, DDP）化疗对人食管癌EC9706细胞增殖和凋亡的影响。方法:采用MTT法分别检测单用ECRG2蛋白和ECRG2蛋白联合顺铂对EC9706细胞增殖的影响；采用Hoechst 33258染色法检测二者对EC9706细胞凋亡的影响；Western blotting检测二者对EC9706细胞中p53蛋白表达的影响。结果:MTT结果显示，ECRG2蛋白可抑制EC9706细胞增殖，ECRG2蛋白和DDP联用后抑制细胞增殖作用增强，且在一定浓度范围内呈时间、剂量依赖关系。Hoechst 33258染色显示，ECRG2蛋白和DDP联合作用24 h后EC9706细胞凋亡数目多于单用ECRG2蛋白。Western blotting结果提示ECRG2蛋白和DDP联合用药较单用ECRG2蛋白明显上调p53蛋白的表达。结论: DDP 可增强ECRG2蛋白对人食管癌EC9706细胞的增殖抑制和凋亡诱导作用，其增强诱导EC9706细胞凋亡的机制可能与上调p53蛋白的表达有关。

Cisplatin enhances proliferation-inhibitory and apoptosis-inducing effects of ECRG2 protein on human esophageal cancer EC9706 cells

Abstract: AIM: To investigate the effects of esophageal cancer-related gene 2 (ECRG2) protein combined with cisplatin (DDP) on the proliferation and apoptosis of human esophageal cancer EC9706 cells. METHODS: Methylthiazolyl tetrazolium (MTT) assay was used to examine the effects of single ECRG2 protein and ECRG2 protein combined with DDP on the proliferation of EC9706 cells. Hoechest 33258 staining was applied to analyze the effect of single ECRG2 protein and ECRG2 protein combined with DDP on apoptosis of EC9706 cells. The expression of p53 in EC9706 cells was detected by Western blotting. RESULTS: ECRG2 protein inhibited the proliferation of EC9706 cells. ECRG2 protein combined with DDP enhanced the inhibitory effect time- and dose-dependently in a certain range of concentrations. The number of apoptotic cells after treated with ECRG2 protein combined with DDP for 24 h was larger than those treated with single ECRG2 protein. The proliferation-inhibitory and qpoptosis-inducing expression of p53 was up-regulated in ECRG2 protein and DDP combination group compared with single ECRG2 protein group. CONCLUSION: DDP enhances the proliferation-inhibitory and apoptosis-inducing effects of ECRG2 protein on EC9706 cells. The apoptosis-inducing mechanism may be related to the up-regulation of p53 expression.