| 人外周血淋巴细胞热应激蛋白70的流式细胞术检测 |
| |
| 引用本文： | 肖成峰,陈胜,潘勤,柯磊,刘藏,王瑞波,高雅娟,贺涵贞,邬堂春.人外周血淋巴细胞热应激蛋白70的流式细胞术检测[J].中华劳动卫生职业病杂志,2001,19(1):59-61. |
| |
| 作者姓名： | 肖成峰 陈胜 潘勤 柯磊 刘藏 王瑞波 高雅娟 贺涵贞 邬堂春 |
| |
| 作者单位： | 1. 华中科技大学同济医学院职业医学研究所
2. 武警南京医院 |
| |
| 基金项目： | 国家自然科学基金青年基金资助项目(39700118);霍英东青年教师基金资助项目(71041) |
| |
| 摘 要： | 目的 建立一种操作简单、快速而又可靠性强的检测细胞热应激蛋白（HSPs）表达水平的方法。方法 采用流式细胞术和免疫印迹方法检测人外周血淋巴细胞热应激蛋白70（HSP70）。结果 外周血淋巴细胞在43℃受热应激30min后，HSP70水平迅速升高，6h达到峰值，之后逐渐降低至正常水平。两种方法所得结果具有高度一致性，但流式细胞术操作简单、省时，可测样本量大，结果可靠，因此更适合于大样本快速研究。结论 运用流式细胞术大规模检测人外周血淋巴细胞HSP70是可行的。
|
| 关 键 词： | 淋巴细胞 热休克蛋白质 流式细胞术 免疫印迹 热应激蛋白 |
| 修稿时间： | 2000年5月23日 |
Detection of heat stress protein 70 in peripheral blood lymphocytes by flow cytometry |
| |
| XIAO Chengfeng ,CHEN Sheng,PAN Qin,et al..Detection of heat stress protein 70 in peripheral blood lymphocytes by flow cytometry[J].Chinese Journal of Industrial Hygiene and Occupational Diseases,2001,19(1):59-61. |
| |
| Authors: | XIAO Chengfeng CHEN Sheng PAN Qin |
| |
| Institution: | XIAO Chengfeng *,CHEN Sheng,PAN Qin,et al. Institute of Occupational Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
| |
| Abstract: | Objective To establish a simple,rapid and r eliable method for evaluating heat stress protein 70(HSP70) in peripheral blood lymphocytes. Methods Flow cytometry and Western blot were used in this study. Results HSP70 in lymphocytes increas ed rapidly after heat stress at 43 ℃ for 30 min,and the peak value of HSP70 app eared at 6 h.Then the level decayed.Similar results of both flow cytometry and W estern blot showed a high coincidence of the two methods.Flow cytometry,however, was more simple,rapid,reliable and suitable for study of large samples. Conclusions Flow cytometry is an accessible method with large-sc ale applicability to detect HSP70 in peripheral blood lymphocytes. |
| |
| Keywords: | Lymphocytes Heat stress proteins Flow cytometry Western blot |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
